Effect of -1064,-1625 SNP on MD-2 promoter activity

SHAN You-an; LIU Qing; DONG Hong; GU Wei; JIANG Jian-xin

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Effect of -1064,-1625 SNP on MD-2 promoter activity

更新时间：2025-01-15

- Effect of -1064,-1625 SNP on MD-2 promoter activity
- Journal of Army Medical University Vol. 29, Issue 3, Pages: 185-187(2007)
- 作者机构：
  
  第三军医大学大坪医院野战外科研究所第四研究室，创伤、烧伤与复合伤国家重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394.3;R631.02;R394-33
- Published：2007，
  
  Published Online：15 February 2007，
- 稿件说明：
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SHAN YOU-AN, LIU QING, DONG HONG, et al. Effect of -1064,-1625 SNP on MD-2 promoter activity. [J]. Journal of army medical university, 2007, 29(3): 185-187.
DOI：

SHAN YOU-AN, LIU QING, DONG HONG, et al. Effect of -1064,-1625 SNP on MD-2 promoter activity. [J]. Journal of army medical university, 2007, 29(3): 185-187. DOI：

摘要

目的探讨MD-2基因启动子区-1064、-1625 SNP位点碱基变异对启动子活性的影响。方法通过PCR和定点突变技术，构建了含MD-2基因基础启动子及-1625 G和-1064 G突变启动子，以双荧光素酶报告系统观察SNP对MD-2基因启动子活性的影响。结果化学发光结果显示，在U937细胞中，-1625 G突变启动子活性比MD-2基础启动子活性高11.4%，差异显著。而-1064 G 突变启动子与MD-2基础启动子活性无明显差异。结论 -1625 C→G碱基变异可增强MD-2基因启动子活性，但-1064 SNP对靶基因启动子活性无明显影响。

Abstract

Objective To study the effect of -1064

-1625 single nucleotide polymorphisms (SNPs) on MD-2 promoter activity. Methods PCR and site direct mutation technology were used to construct MD-2 basic promoter and -1064 G

-1625 G mutate promoter. Dual-Luciferase Reporter assay system was used to detect the activity of constructed promoter. Results In U937 cells

the activity of -1625 G mutate promoter was higher than -1625C promoter

and there was no significant difference between -1064A promoter and -1064 G promoter activity. Conclusion It is implied that -1625 C→G base variation can enhance the activity of MD-2 promoter

while -1064 SNP has no effect on MD-2 promoter activity.

关键词

MD-2启动子单核苷酸多态性

Keywords

MD-2promotersingle nucleotide polymorphisms

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