Objective     To observe the effect of aerosolized perfluorocarbon (PFC) (FC77) and ambroxol on gas exchange

histopathological changes of lung after acute injury

pulmonary expression of tumor necrosis factor-α mRNA (TNF-α mRNA) and antioxidation.      Methods     After acute lung injury (ALI) was induced by oleic acid (OA)

thirty-two rabbits were assigned randomly to 4 groups: conventional mechanical ventilation group (A)

aerosolize

d PFC group (B)

aerosolized ambroxol group (C) and combined aerosolization group(D). The changes of gas exchange were examined before ALI

during ALI and at 1st

2nd

3rd

4th h after ALI

and histological sections taken from 6 different parts of lung were stained by HE. The expression of TNF-α mRNA in the 2 different parts of lung were detected by in situ hybridization (ISH)

malondialdehyde (MDA) levels in lung homogenate was determined by the thiobarbituric acid (TBA) reaction.      Results     The PaO

2

and static lung compliance (CLst) in group D significantly increased compared with group A (

P

<

0.05)

but showed no significant difference with group B. The histopathological lesions of lung were less severe in group D than group A. The TNF-α mRNA expression significantly decreased in group D as compared with groups A and B (

P

<

0.05)

and was more in backside of lung than foreside of lung in four groups (

P

<

0.05). The MDA levels in lung tissues decreased in group D compared with groups A and B (

P

<

0.05).       Conclusion     The combined aerosolization of perfluorocarbon (PFC) and ambroxol can decrease tumor necrosis factor-α mRNA expression

lessen the injury caused by free radical in lung

improve the CLst and oxygenation of lung

increase PaO

2

during acute lung injury.