Effect of melatonin on GAD67 mRNA expression in post-SE rat hippocampus induced by pilocarpine

ZHANG Ying-qi; LIAO Wei-hong; LONG Zai-yun

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Effect of melatonin on GAD67 mRNA expression in post-SE rat hippocampus induced by pilocarpine

更新时间：2025-01-15

- Effect of melatonin on GAD67 mRNA expression in post-SE rat hippocampus induced by pilocarpine
- Journal of Army Medical University Vol. 29, Issue 16, Pages: 1605-1608(2007)
- 作者机构：
  
  第三军医大学：西南医院神经内科，大坪医院野战外科研究所第三研究室，创伤、烧伤与复合伤国家重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R338.26;R749.17;R977.1
- Published：2007，
  
  Published Online：30 August 2007，
- 稿件说明：
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ZHANG YING-QI, LIAO WEI-HONG, LONG ZAI-YUN. Effect of melatonin on GAD67 mRNA expression in post-SE rat hippocampus induced by pilocarpine. [J]. Journal of army medical university, 2007, 29(16): 1605-1608.
DOI：

ZHANG YING-QI, LIAO WEI-HONG, LONG ZAI-YUN. Effect of melatonin on GAD67 mRNA expression in post-SE rat hippocampus induced by pilocarpine. [J]. Journal of army medical university, 2007, 29(16): 1605-1608. DOI：

摘要

目的从神经递质角度探讨褪黑素（melatonin

Mel）抗癫痫作用的机制。方法采用匹罗卡品（pilocarpine

PILO）诱发大鼠癫痫持续状态（status epilepticus

SE）模型，观察大鼠SE后4个时相点即6、48、72 h和7 d海马GABA含量和GAD67 mRNA表达的变化，以及Mel对其变化的影响。结果 PILO诱导大鼠SE后6 h至7 d，海马GABA含量显著降低，与对照组比较差异极显著（P<0.01），GAD67 mRNA的表达于SE后6~72 h较对照组明显降低（P<0.01），SE后7 d，GAD67 mRNA的表达升高并明显高于对照组（P<0.05）；PILO+Mel组大鼠SE后6 h至7 d，海马GABA水平显著高于PILO组（P<0.01），并且GAD67 mRNA的表达在SE后6~72 h，也显著高于PILO组大鼠（P<0.05）。结论 Mel可能通过上调GAD67 mRNA的表达，使GABA合成增加，抑制功能增强来发挥抗癫痫作用。

Abstract

Objective To probe the mechanism of anticonvulsant by melatonin from the angle of neurotransmitter. Methods Rat status epilepticus (SE) model was induced by pilocarpine(PILO). γ-aminobutyric acid (GABA) content and glutamin acid decarboxylase(GAD)67 mRNA expression was detected at 6

72 h

and 7 d in the hippocampus of post-SE rats. The effect of melatonin on these changes was observed. Results GABA content was significantly lower in the hippocampus than in control（P<0.01）. At 6-72 h post-SE

the expression of GAD67 mRNA was significantly lower in PILO group than in control（P<0.01）

but significantly higher in PILO group than in control at 7 d post-SE. The content of GABA was significantly higher in PILO+Mel group than in PILO group at 6 h-7 d post-SE（P<0.01）and the expression of GAD67 mRNA was significantly higher in PILO+Mel group than in PILO group at 6-72 h post-SE (P<0.05). Conclusion Anticonvulsive effect of melatonin may be due to its ability to increase the expression of GAD67 mRNA

so to increase the content of GABA.

关键词

癫痫持续状态海马褪黑素γ-氨基丁酸谷氨酸脱羧酶

Keywords

status epilepticushippocampusmelatoninγ-aminobutyric acidglutamin acid decarboxylase

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