Molecular mechanism of invasion inhibitory effects of genistein on human ovarian serous papillary cystadenocarcinoma cell SKOV<sub>3</sub>

LI Yu; MI Can

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Molecular mechanism of invasion inhibitory effects of genistein on human ovarian serous papillary cystadenocarcinoma cell SKOV₃

更新时间：2025-01-15

- Molecular mechanism of invasion inhibitory effects of genistein on human ovarian serous papillary cystadenocarcinoma cell SKOV₃
- Journal of Army Medical University Vol. 29, Issue 16, Pages: 1601-1604(2007)
- 作者机构：
  
  重庆医科大学基础医学院病理学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R73-354;R979.19;R737.31
- Published：2007，
  
  Published Online：30 August 2007，
- 稿件说明：
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LI YU, MI CAN. Molecular mechanism of invasion inhibitory effects of genistein on human ovarian serous papillary cystadenocarcinoma cell SKOV₃. [J]. Journal of army medical university, 2007, 29(16): 1601-1604.
DOI：

LI YU, MI CAN. Molecular mechanism of invasion inhibitory effects of genistein on human ovarian serous papillary cystadenocarcinoma cell SKOV₃. [J]. Journal of army medical university, 2007, 29(16): 1601-1604. DOI：

摘要

目的探讨 genistein 抑制人卵巢浆液性乳头状囊腺癌细胞系 SKOV

体外侵袭作用的分子机制。方法利用微孔滤膜培养小室进行双室联合培养，建立体外趋化运动模型，观察 genistein 对SKOV

趋化运动能力的影响；用免疫组化和原位杂交方法检测 genistein 对细胞表面黏附分子CD44v6、基质金属蛋白酶及其组织抑制物MMP-9/TIMP-1，MMP-2/TIMP-2蛋白和mRNA表达水平的影响。结果 20 μmol/L和40 μmol/L genistein处理SKOV

细胞后，细胞的运动能力分别下降至对照组的（46.9±5.8）%和（28.3±4.7）%，差异显著（P

0.01）。免疫细胞化学和原位杂交检测结果表明：和对照组相比，20 μmol/L genistein处理SKOV

细胞后48 h，CD44v6、 MMP-9及MMP-2的蛋白和mRNA表达均减弱（P

0.05），而TIMP-1和TIMP-2的蛋白和mRNA表达水平增强（P

0.05）。结论 genistein明显抑制SKOV

细胞的趋化运动能力、降低SKOV

细胞黏附分子CD44v6的表达、下调MMP-9与MMP-2表达，并上调TIMP-1与TIMP-2表达，这可能是其抑制人卵巢癌细胞系SKOV

体外侵袭作用的重要分子基础

Abstract

Objective To investigate how genistein to inhibit the invasion of human ovarian serous papillary cystadenocarcinoma cell line SKOV

. Methods Millicell chamber and coculture method were used to establish chemotactic migration model in vitro to observe the effect of genistein on directional che

motactic migration movement of SKOV

cells. The protein expressions of cell surface adhesion molecule CD44v6

matrix metalloproteinases and tissue inhibitor of metalloproteinases MMP-9/TIMP-1 and MMP-2/TIMP-2 were determined by using immunocytochemical method and their mRNA levels were examined by in situ hybridization. Results Compared with control group

the directional chemotactic migration of SKOV

were significantly decreased after treated with 20 μmol/L and 40 μmol/L genistein

reached to (46.9±5.8)% and (28.3±4.7)% respectively (P

0.01). genistein at 20 μmol/L down-regulated the protein and mRNA expressions of CD44v6

MMP-9 and MMP-2 (P

0.05)

up-regulated those of TIMP-1 and TIMP-2 (P

0.05). Conclusion genistein exerts inhibitory effects on the invasion of SKOV

cells by inhibiting chemotactic migration of the cells

that is

down-regulating the protein and mRNA expressions of CD44v6

MMP-9 and MMP-2

and up-regulating TIMP-1 and TIMP-2 expressions.

关键词

genistein卵巢肿瘤侵袭

Keywords

genisteinovarian tumorinvasion

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Molecular mechanism of invasion inhibitory effects of genistein on human ovarian serous papillary cystadenocarcinoma cell SKOV3

DOI：

摘要

Abstract

关键词

Keywords

references

Molecular mechanism of invasion inhibitory effects of genistein on human ovarian serous papillary cystadenocarcinoma cell SKOV₃