Decreased brain-derived neurotrophic factor in cortex, hippocampus and cerebrospinal fluid of depressed rats

YIN Lie-hu; LI Xue-cheng; LU Di; JIANG Yu; WU Hong-chen; YANG Zhong; TIAN Jing; WU Xi-gui

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Decreased brain-derived neurotrophic factor in cortex, hippocampus and cerebrospinal fluid of depressed rats

更新时间：2025-01-15

- Decreased brain-derived neurotrophic factor in cortex, hippocampus and cerebrospinal fluid of depressed rats
- Journal of Army Medical University Vol. 31, Issue 3, Pages: 230-232(2009)
- 作者机构：
  
  1. 解放军第324医院精神病科
  2. 第三军医大学基础医学部神经生物学教研室，重庆市神经科学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R338;R749.4;R322.8
- Published：2009，
  
  Published Online：15 February 2009，
- 稿件说明：
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YIN LIE-HU, LI XUE-CHENG, LU DI, et al. Decreased brain-derived neurotrophic factor in cortex, hippocampus and cerebrospinal fluid of depressed rats. [J]. Journal of army medical university, 2009, 31(3): 230-232.
DOI：

YIN LIE-HU, LI XUE-CHENG, LU DI, et al. Decreased brain-derived neurotrophic factor in cortex, hippocampus and cerebrospinal fluid of depressed rats. [J]. Journal of army medical university, 2009, 31(3): 230-232. DOI：

摘要

目的观察抑郁对大鼠脑脊液、海马和皮层组织脑源性神经营养因子(brain-derived neurotrophic factor

BDNF)的影响，探讨BDNF与抑郁症的发病关系及作用机制。方法采用慢性不可预见性应激法制作大鼠抑郁模型

运用Open-Field评分方法观察大鼠行为学变化；免疫组化法检测海马和皮层BDNF的表达，ELISA法检测脑脊液、海马和皮层BDNF的含量。结果与正常对照组比较，抑郁模型组大鼠海马和皮层BDNF阳性细胞数量均明显减少；正常对照组大鼠脑脊液、海马和皮层BDNF的含量分别为:（6.87±2.44）ng/ml、（11.75±3.60）ng/g、（4.31±1.37）ng/g，抑郁模型组大鼠脑脊液、海马和皮层BDNF的含量分别为：（4.23±1.54）ng/ml、（8.56±2.45）ng/g、（3.06±0.67）ng/g，表明抑郁模型组大鼠脑组织中BDNF的含量显著低于正常对照组（

＜0.01）。结论抑郁模型大鼠脑组织中BDNF水平降低可能是导致抑郁症发生的重要因素之一。

Abstract

Objective To explore the effect of depression on the expression of brain-derived neurotrophic factor (BDNF) in the cerebral cortex

hippocampus and cerebrospinal fluid in order to investigate the relationship between BDNF and the occurrence of depression. Methods The depression model in rats was established with chronic unpredictable stress. The behaviors in Open-Field testing were observed through forced swimming. The expression of BDNF in above-mentioned tissues was examined by immunohistochemical method and ELISA. Results Compa

red with normal control

the number of BDNF positive cells in the hippocampus and cerebral cortex in depressed rats was decreased markedly. The level of BDNF in the cerebral-spinal fluid

hippocampus and cerebral cortex in normal mice was (6.87±2.44)ng/ml

(11.75±3.60)ng/g and (4.31±1.37)ng/g respectively; and they were markedly higher than those of depressed rats [(4.23±1.54)ng/ml

(8.56±2.45)ng/g and (3.06±0.67)ng/g

0.01

]

. Conclusion BDNF levels are obviously decreased in the depressed rats

indicating it may be involved in the occurrence of depression.

关键词

慢性应激抑郁脑源性神经营养因子ELISA

Keywords

chronic stressdepressionbrain-derived neurotrophic factorELISA

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