Effects of activin and follistatin on hydroxyproline and type Ⅳ collagen secreted by cultured rat renal interstitial fibroblasts

LI Fu-rong; YANG Cheng; ZHANG Ying; YUAN Fa-huan

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Effects of activin and follistatin on hydroxyproline and type Ⅳ collagen secreted by cultured rat renal interstitial fibroblasts

更新时间：2025-01-15

- Effects of activin and follistatin on hydroxyproline and type Ⅳ collagen secreted by cultured rat renal interstitial fibroblasts
- Journal of Army Medical University Vol. 31, Issue 3, Pages: 238-240(2009)
- 作者机构：
  
  第三军医大学新桥医院肾内科，全军肾脏病中心，重庆市肾病研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.26;R361.3;R692.05
- Published：2009，
  
  Published Online：15 February 2009，
- 稿件说明：
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LI FU-RONG, YANG CHENG, ZHANG YING, et al. Effects of activin and follistatin on hydroxyproline and type Ⅳ collagen secreted by cultured rat renal interstitial fibroblasts. [J]. Journal of army medical university, 2009, 31(3): 238-240.
DOI：

LI FU-RONG, YANG CHENG, ZHANG YING, et al. Effects of activin and follistatin on hydroxyproline and type Ⅳ collagen secreted by cultured rat renal interstitial fibroblasts. [J]. Journal of army medical university, 2009, 31(3): 238-240. DOI：

摘要

目的研究激活素A(activin A，ACT-A)及卵泡抑素(follistatin，FS)在肾间质纤维化中的作用。方法 SD大鼠肾间质成纤维细胞体外培养后分为A组、F组、A+F组，分别向培养液中加入ACT-A、FS、ACT-A+FS，用ELISA法检测培养液上清中羟脯氨酸(hydroxyproline，HYP)及Ⅳ型胶原(Ⅳ-C)的含量。结果在本研究浓度范围内，ACT-A浓度越高，培养液中HYP及Ⅳ-C浓度也越高;单纯加入FS对大鼠肾间质成纤维细胞分泌HYP及Ⅳ-C没有显著影响（

>0.05），但以剂量依赖方式抑制ACT-A促大鼠肾间质成纤维细胞分泌HYP及Ⅳ-C的作用（

0.01）。结论 ACT-A可能在肾间质损害的发生、发展中起重要作用，补充外源性的FS有可能为治疗肾间质纤维化提供新的思路。

Abstract

Objective To investigate the roles of activin A (ACT-A) and follistatin (FS) in renal interstitial fibrosis. Methods Renal interstitial fibroblasts were isolated from SD rat kidney and primarily cultured. The cells were divided into A group

F group and A+F group

and then each group was further divided into 5 subgroups according to their culture media being added with ACT-A (0

0.3

or 100 ng/ml)

FS (0

0.3

or 100 ng/ml)

and ACT-A (30 ng/ml) plus FS (0

0.3

or 100 ng/ml) respectively. Levels of type Ⅳ collagen (Ⅳ-C) and hydroxyproline (HYP) in the supernatants of cultured fibroblasts were measured by ELISA assay. Results Concentrations of Ⅳ-C and HYP in cultured rat renal interstitial fibroblasts were in a dose-dependent manner with ACT-A treatment. FS had no effect on these concentrations

but it inhibited the effects of ACT-A on Ⅳ-C and HYP in the supernatants of cultured fibroblasts in a dose-dependent manner. Conclusion ACT-A might be involved in occurrence and development of renal fibrosis by affecting renal interstitial fibroblasts. Exogenous FS could suppress the effects of ACT-A on the cultured renal fibroblasts.

关键词

激活素A卵泡抑素成纤维细胞Ⅳ型胶原羟脯氨酸

Keywords

activin Afollistatinfibroblaststype Ⅳ collagenhydroxyproline

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