Effect of small interfering RNA against connective tissue growth factor in TGF-β<sub>1</sub>stimulated human keloid fibroblasts

ZHAO Ping; LI Shi-rong

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Effect of small interfering RNA against connective tissue growth factor in TGF-β₁stimulated human keloid fibroblasts

更新时间：2025-01-15

- Effect of small interfering RNA against connective tissue growth factor in TGF-β₁stimulated human keloid fibroblasts
- Journal of Army Medical University Vol. 31, Issue 3, Pages: 213-215(2009)
- 作者机构：
  
  第三军医大学西南医院整形外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.28;R619.6;Q813.1
- Published：2009，
  
  Published Online：15 February 2009，
- 稿件说明：
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ZHAO PING, LI SHI-RONG. Effect of small interfering RNA against connective tissue growth factor in TGF-β₁stimulated human keloid fibroblasts. [J]. Journal of army medical university, 2009, 31(3): 213-215.
DOI：

ZHAO PING, LI SHI-RONG. Effect of small interfering RNA against connective tissue growth factor in TGF-β₁stimulated human keloid fibroblasts. [J]. Journal of army medical university, 2009, 31(3): 213-215. DOI：

摘要

目的体外构建结缔组织生长因子（CTGF）序列特异性小干扰RNA(small interfering RNA

siRNA)表达载体

研究其对人瘢痕疙瘩成纤维细胞CTGF表达基因和蛋白的影响。方法体外构建CTGF序列特异性siRNA质粒表达载体

Dosper脂质体转染人瘢痕疙瘩成纤维细胞后，用10 ng/ml剂量的转化生长因子（TGF-β

）刺激细胞，采用RQ-PCR和Western blot观察CTGF mRNA和蛋白水平的变化。结果经TGF-β

刺激后，重组质粒组CTGF mRNA仅为正常表达的56.6％（

0.05），CTGF蛋白仅为正常表达的基线水平（

0.05）。结论 siRNA质粒表达载体可抑制瘢痕疙瘩成纤维细胞CTGF基因表达和蛋白分泌，即使在TGF-β

刺激条件下，其分泌功能仍明显受抑。

Abstract

Objective To investigate the effect of small interfering RNA (siRNA)-expressing plasmid targeting connective tissue growth factor (CTGF) on the expressions of its mRNA and protein in cultured human keloid fibroblasts (hKFs). Methods Constructed siRNA-expressing plasmids targeting CTGF were transfected into hKFs by Dosper package. Then the transfected cells were treated with transforming growth factor 1 (TGF-β

10 ng/ml). Normal hKFs cells and those with TGF-β

treatment served as normal control and control groups respectively.

The expression level of CTGF mRNA and protein were detected by real-time fluorescent quantitative PCR (RQ-PCR) and Western blotting respectively. Results The expression level of CTGF mRNA was only 56.6% of that of the normal control(

0.05)

and the level of CTGF protein was close to that of the normal control(

0.05). Conclusion Our constructed CTGF siRNA plasmid suppresses the mRNA and protein expressions of the target gene in cultured hKFs

even in the cells after TGF-β

stimulation. This approach may be used as a novel method to treat keloid by gene therapy.

关键词

结缔组织生长因子RNA干扰人瘢痕疙瘩成纤维细胞转化生长因子

Keywords

connective tissue growth factorRNA interferinghuman keloid fibroblaststransforming growth factor

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Related Author

ZHU Qi

JIANG Yi

WAN Ping

BAI Ganping

ZHANG Ronghua

DONG Anying

CAI Xiuhua

XU Pei-rong

Related Institution

Department of Heart, Kidney and Respiratory Diseases, No.951 Hospital of PLA, Korla, Xinjiang Uygur Autonomous Region

Department of Traditional Chinese Medicine and Rheumatology, First Affiliated Hospital, Army Medical University (Third Military Medical University)

Department of Pediatrics, The First Affiliated Hospital, Henan Key Laboratory of Molecular Medicine, Zhengzhou University

Department of General Surgery, Three Gorges Central Hospital

Department of General Surgery, Chongqing Emergency Center

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Effect of small interfering RNA against connective tissue growth factor in TGF-β1stimulated human keloid fibroblasts

DOI：

摘要

Abstract

关键词

Keywords

references

Effect of small interfering RNA against connective tissue growth factor in TGF-β₁stimulated human keloid fibroblasts