A protein chip detecting 5 antibodies against <em>mycobacterium tuberculosis</em>: evaluation in diagnosis of tuberculosis

XIA Ji; CAI Jin; DENG Shao-li; HUANG Heng-liu; CHEN Wei; YANG Tong-huai

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A protein chip detecting 5 antibodies against mycobacterium tuberculosis: evaluation in diagnosis of tuberculosis

更新时间：2025-01-15

- A protein chip detecting 5 antibodies against mycobacterium tuberculosis: evaluation in diagnosis of tuberculosis
- Journal of Army Medical University Vol. 31, Issue 3, Pages: 220-222(2009)
- 作者机构：
  
  第三军医大学：大坪医院野战外科研究所检验科，西南医院检验科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R378.911;R392.11;R446.11
- Published：2009，
  
  Published Online：15 February 2009，
- 稿件说明：
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XIA JI, CAI JIN, DENG SHAO-LI, et al. A protein chip detecting 5 antibodies against mycobacterium tuberculosis: evaluation in diagnosis of tuberculosis. [J]. Journal of army medical university, 2009, 31(3): 220-222.
DOI：

XIA JI, CAI JIN, DENG SHAO-LI, et al. A protein chip detecting 5 antibodies against mycobacterium tuberculosis: evaluation in diagnosis of tuberculosis. [J]. Journal of army medical university, 2009, 31(3): 220-222. DOI：

摘要

目的探讨结核分枝杆菌抗LAM、38 kD、16 kD、CFP10、ESAT-6 5项抗体的检测对临床的应用价值。方法检测50例结核病、80例非结核病患者血清，应用蛋白芯片识别仪检测受试者血清中5项结核抗体，并对诊断试验结果进行分析评价。结果 130例临床标本显示，以任一抗体检测阳性诊断为结核病阳性，则诊断结核病的灵敏度为92.0%，特异度为77.5%，正确率为83.1%，Youden指数为69.5%。结论蛋白芯片联合检测5项结核抗体具有较高的诊断价值，与“金标准”诊断的一致性较好，且取材简易、操作方便、历时较短，可以作为临床上诊断结核病特别是肺外结核病的一项重要辅诊项目。

Abstract

Objective To detect 5 antibodies against tuberculoprotein

LAM

CFP10

ESAT-6 and 2 proteins at molecular weight of 38 kD and 16 kD by a protein chip and explore its clinical value in diagnosis of tuberculosis. Methods The serum antibodies from 50 tuberculosis patients and 80 non-tuberculosis patients were analyzed by this protein chip

and the diagnostic value of the test was evaluated. Results As was shown in the 130 clinical samples

the sensitivity

specificity

accuracy and Youden index of the diagnostic test were 92.0%

77.5%

83.1% and 69.5% respectively. Conclusion The tuberculosis protein chip assay is of high value in diagnosis of both pulmonary and non-pulmonary tuberculosis patients

and has good coincidence with gold standard. It is time-saving and easy to sampling and practicing

and can be regarded as supplemental item for non-pulmonary tuberculosis.

关键词

结核分枝杆菌结核抗体蛋白芯片

Keywords

mycobacterium tuberculosisantibodiesprotein chip

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Related Institution

Department of Infectious Diseases， Key Laboratory of Children Development of Ministry of Education, Chongqing Key Laboratory of Pediatrics, Children’s Hospital of Chongqing Medical University

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A protein chip detecting 5 antibodies against mycobacterium tuberculosis: evaluation in diagnosis of tuberculosis

DOI：

摘要

Abstract

关键词

Keywords

references