Simultaneously determining 4 amino acid neurotransmitters in mice brain by high-performance liquid chromatography with fluorescence detector

GUI Li; TIAN Hong; ZHENG Jian; DUAN Wei; YE Feng

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Simultaneously determining 4 amino acid neurotransmitters in mice brain by high-performance liquid chromatography with fluorescence detector

更新时间：2025-01-15

- Simultaneously determining 4 amino acid neurotransmitters in mice brain by high-performance liquid chromatography with fluorescence detector
- Journal of Army Medical University Vol. 31, Issue 8, Pages: 675-678(2009)
- 作者机构：
  
  第三军医大学：新桥医院神经内科,军事预防医学院防化医学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R345.17;R446.1;R322.81
- Published：2009，
  
  Published Online：30 April 2009，
- 稿件说明：
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GUI LI, TIAN HONG, ZHENG JIAN, et al. Simultaneously determining 4 amino acid neurotransmitters in mice brain by high-performance liquid chromatography with fluorescence detector. [J]. Journal of army medical university, 2009, 31(8): 675-678.
DOI：

GUI LI, TIAN HONG, ZHENG JIAN, et al. Simultaneously determining 4 amino acid neurotransmitters in mice brain by high-performance liquid chromatography with fluorescence detector. [J]. Journal of army medical university, 2009, 31(8): 675-678. DOI：

摘要

目的采用高效液相-荧光检测技术建立一种稳定的用于测定脑组织中多种氨基酸类神经递质的方法。方法用微透析技术收集小鼠脑组织中多种氨基酸类神经递质，用邻苯二甲醛(OPA)柱前衍生高效液相色谱（high performance liquid chromatography，HPLC）法测定微透析液中天门冬氨酸（Asp）、谷氨酸(Glu)、甘氨酸(Gly)、γ-氨基丁酸(GABA)的含量。结果兴奋性Asp、Glu及抑制性Gly、GABA在18 min内完全分离，在0.625～5 μmol/L浓度范围内与峰面积有良好的线性关系。结论 HPLC法快速、准确、灵敏度高，适用于脑组织中氨基酸类神经递质含量的测定。

Abstract

Objective To establish a stable method of HPLC-FL to determine multi-amino acids in the mice microdialysate. Methods The sample was collected by microdialysis. Four amino acid neurotransmitters

Asp

Glu

Gly and GABA in the microdialysate were determined by reverse phase HPLC with fluorescence detection after o-Phthaldehyde (OPA) pre-column derivatization. Results Excitatory amino acids

Asp and Glu

and inhibitory amino acids

Gly and gamma-aminobutyric acid (GABA) were totally separated and determined within 18 min. A good linear response was obtained for the 4 standards over the range of 0.625 to 5 μmol/L with correlation coefficients. Conclusion Our method is quick

reliable and sensitive to measure the amino acid neurotransmitters in mice brain.

关键词

高效液相色谱柱前衍生微透析氨基酸神经递质

Keywords

high performance liquid chromatographyprecolumn derivatizationmicrodialysisamino acidneurotransmitters

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