Objective    To explore the effect of pyrrolidine d

ithiocarbamate (PDTC) on the expression of E-selectin and the activities of enzymes in oxidative stress in the pancreas of rats with severe acute pancreatitis (SAP).     Methods    Rat model of ASP was build by intraperitoneal injection of L-arginine (totally 5 g/kg

twice in an interval of 1 h). Thirty-six SD rats were assigned to 6 groups randomly and equally

that is

normal control

SAP

PDTC pretreatment group 1 (1 mg/kg) and 2 (100 mg/kg)

and PDTC treatment group 1 (1 mg/kg) and 2 (100 mg/kg). PDTC pretreatment was preformed by subcutaneous injection of PDTC 1 h before first injection of L-arginine. For the animals in treatment groups

PDTC was given 3 h after first injection. Normal control was given intraperitoneal injection of normal saline. In 24 h after the first injection of L-arginine

the activity of myeloperoxidase (MPO)

lipid peroxide (LPO) and nitric oxide synthase (NOS) were detected. Expressions of E-selectin was detected through inmmunohistochemical method

and activity of NF-κB-DNA binding were detected by electrophoretic mobility shift assay (EMSA).     Results    In SAP groups and PDTC pretreatment and treatment groups

the expression of E-selectin

activities of NF-κB

MPO

LPO

and inducible NOS (iNOS) were higher

but the activity of constitutive NOS (cNOS) was lower than those in the normal control (

P

<

0.05). PDTC treatment group 1 had comparatively lower above increased items

but higher activity of cNOS in comparison with SAP group (

P

<

0.05)

and PDTC pretreatment group 1 was more significant (

P

<

0.01). However

PDTC at the dosage of 100 mg/kg no matter before or after L-arginine injection had few effects on all above items compared to SAP group (

P

>0.05).     Conclusion    PDTC of 1 mg/kg can alleviate the inflammation in SAP by blocking the translocation of NF-κB

however

the 100 mg/kg PDTC shows little protection in this process.