Role of bcl-2 in human vascular endothelial cell apoptosis induced by oxidized low density lipoprotein <em>in vitro</em>

CHEN Chun-ye; ZHANG Qian-yong; YI Long; JIN Xin; CHEN Ka; LU Jing; MI Man-tian

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Role of bcl-2 in human vascular endothelial cell apoptosis induced by oxidized low density lipoprotein in vitro

更新时间：2025-01-15

- Role of bcl-2 in human vascular endothelial cell apoptosis induced by oxidized low density lipoprotein in vitro
- Journal of Army Medical University Vol. 31, Issue 8, Pages: 663-667(2009)
- 作者机构：
  
  第三军医大学军事预防医学院营养与食品卫生学教研室,重庆市营养与食品安全重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.12;R329.25;R341.35
- Published：2009，
  
  Published Online：30 April 2009，
- 稿件说明：
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CHEN CHUN-YE, ZHANG QIAN-YONG, YI LONG, et al. Role of bcl-2 in human vascular endothelial cell apoptosis induced by oxidized low density lipoprotein in vitro. [J]. Journal of army medical university, 2009, 31(8): 663-667.
DOI：

CHEN CHUN-YE, ZHANG QIAN-YONG, YI LONG, et al. Role of bcl-2 in human vascular endothelial cell apoptosis induced by oxidized low density lipoprotein in vitro. [J]. Journal of army medical university, 2009, 31(8): 663-667. DOI：

摘要

目的观察氧化低密度脂蛋白（oxidized low density lipoprotein

oxLDL）对人血管内皮细胞株

EA.hy

926凋亡的影响，并探讨bcl-2在其中的作用。方法 MTT法检测不同浓度的oxLDL（50、100、150、200 μg/ml）对细胞增殖能力的影响；流式细胞仪和激光共聚焦显微镜检测不同浓度的oxLDL对细胞凋亡的影响；RT-PCR、Western blot和免疫荧光细胞化学法分别检测不同浓度的oxLDL对bcl-2 mRNA、蛋白表达及在细胞内表达的影响。结果 oxLDL对

EA.hy

926细胞形态有明显影响，并能明显抑制EA.hy926细胞增殖能力，抑制作用随处理浓度增加而增大，半数抑制浓度（IC

）约为100 μg/ml；不同浓度的oxLDL处理细胞24 h后，与对照组比较，早期凋亡、晚期凋亡和总凋亡细胞百分比均显著增加(

0.05)；oxLDL 能够明显抑制细胞内bcl-2 mRNA、蛋白表达及在细胞内的表达水平。结论 bcl-2基因在oxLDL诱导的血管内皮细胞凋亡中发挥重要作用，oxLDL可能通过下调bcl-2的表达促进血管内皮细胞凋亡。

Abstract

Objective To study the effect of bcl-2 in apoptosis of human vascular endothelial cells line

EA.hy

926 induced by oxidized low density lipoprotein (oxLDL). Methods MTT assay was used to detect the effect of oxLDL at different concentrations of 50

100

150 and 200 μg/ml on the proliferation of

EA.hy

926 cells. Flow cytometry and laser scanning confocal microscopy were employed to detect the apoptosis of

EA.hy

926 cells after

oxLDL treatment. The expressions of bcl-2 mRNA and protein were observed respectively by RT-PCR

Western blotting and immunofluorescence cytochemistry. Results After exposed to oxLDL at different concentrations

the morphology of

EA.hy

926 cells turned to be unbright

irregular in shape

and black granules in the cytoplasm. The proliferation was inhibited significantly in a dose-dependent manner

and the IC

was 100 μg/ml. The percentage of early

late and total apoptotic cells were all significantly increased after oxLDL treatment compared to the control group (

0.05). The expression of bcl-2 mRNA and protein were both downregulated (

0. 05) by oxLDL. Conclusion oxLDL may induce the apoptosis of endothelial cells through downregulating bcl-2 mRNA and protein expression.

关键词

动脉粥样硬化氧化低密度脂蛋白人血管内皮细胞细胞凋亡Bcl-2

Keywords

atherosclerosisoxidized low density lipoproteinhuman vascular endothelial cellsapoptosisBcl-2

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Role of bcl-2 in human vascular endothelial cell apoptosis induced by oxidized low density lipoprotein in vitro

DOI：

摘要

Abstract

关键词

Keywords

references