Role of p38MAPK in substance P-induced activation of spinal cord astrocytes <em>in vitro</em>

WANG Qiang; SONG Bo

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Role of p38MAPK in substance P-induced activation of spinal cord astrocytes in vitro

更新时间：2025-01-15

- Role of p38MAPK in substance P-induced activation of spinal cord astrocytes in vitro
- Journal of Army Medical University Vol. 31, Issue 8, Pages: 689-693(2009)
- 作者机构：
  
  第三军医大学西南医院全军泌尿外科研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.25;{R392.13};R322.81
- Published：2009，
  
  Published Online：30 April 2009，
- 稿件说明：
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WANG QIANG, SONG BO. Role of p38MAPK in substance P-induced activation of spinal cord astrocytes in vitro. [J]. Journal of army medical university, 2009, 31(8): 689-693.
DOI：

WANG QIANG, SONG BO. Role of p38MAPK in substance P-induced activation of spinal cord astrocytes in vitro. [J]. Journal of army medical university, 2009, 31(8): 689-693. DOI：

摘要

目的观察体外培养脊髓星形胶质细胞P物质刺激活化过程中p38MAPK活性、TNF-α、NO水平及NOS活性的变化，探讨p38MAPK活性变化与脊髓星形胶质细胞活化的关系。方法分离培养的SPF大鼠脊髓星形胶质细胞设正常对照组（正常组）、SP刺激组（SP组，10

-7

mol/L）、SB203580阻断p38MAPK组（SB组，10 μmol/L）和SP刺激+SB203580阻断p38MAPK组（SP+SB组）。WB法、RT-PCR法、ELISA法检测1

24 h和36 h时p38MAPK、p-p38（磷酸化p38MAPK）含量及GFAP mRNA、TNF-α、NO水平和NOS活性的变化。结果脊髓星形胶质细胞GFAP 阳性表达率大于95%。SP组脊髓星形胶质细胞总p38MAPK水平无显著变化，1 h后p-p38开始升高，3 h后GFAP mRNA水平显著增高，同时NO、NOS和TNF-a水平显著增高。用SB203580阻断p38MAPK通路后，SP+SB组较SP组p-p38、GFAP mRNA、NO、NOS、TNF-a水平显著降低。SB组总p38MAPK、p-p38、GFAP mRNA、NO、NOS、TNF-a水平无显著变化。结论 p38MAPK信号通路参与了脊髓星形胶质细胞P物质刺激活化过程，阻断p38MAPK信号通路可有效降低炎性因子水平。

Abstract

Objective To investigate the relationship between p38MAPK and astrocytes activation by detecting the changes of p38MAPK

TNF-α

NO and NOS in the substance P-induced activation of spinal cord astrocytes

in vitro

. Methods Astrocytes

cultured from spinal cord of SPF rat and identified by immunofluorescence method with GFAP antibody

were grouped into the control group

the SP stimulus group (SP group) and the SB20

3580 interrupt p38MAPK group (interrupt group) in which SP (10

-7

mol/L) and SB203580 (10 μmol/L) were added to the supernatant for 12 h. The WB method

RT-PCR and ELISA were used to determine the changes of p38MAPK

phosphorylated p38MAPK

GFAP mRNA

TNF-α

NO and NOS in the astrocytes or supernatant at 1

24 and 36 h respectively. Results The GFAP positive rate of the cultured cells was higher than 95%. p38MAPK level in the SP group did not have any change

whereas p-p38 level increased significantly after 1 h and GFAP mRNA

NOS and TNF-α level increased after 3 h. When p38MAPK pathway was inhibited by SB203580 in the SP+SB group

GFAP mRNA

NOS and TNF-α were significantly reduced compared with those in the SP group. The level of p38MAPK

p-p38

GFAP mRNA

NOS and TNF-α in the SB group did not have changes compared with those in the normal group. Conclusion These results indicate that p38MAPK signal pathway contributes to substance P induced activation of spinal cord astrocytes according to inflammatory factors attenuation after p38MAPK signal pathway interruption by SB203580.

关键词

脊髓星形胶质细胞活化p38MAPK

Keywords

spinal cordastrocytesactivationp38MAPK

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⁰

Role of p38MAPK in substance P-induced activation of spinal cord astrocytes in vitro

DOI：

摘要

Abstract

关键词

Keywords

references