Effect of cimetidine on clonal expansion of TCR Vβ subfamily of T cells in cord blood stimulated by K562 cells

TIAN Hong-xia; LIN Chen; CHEN Shao-hua; YANG Li-jian; JIANG Zhen-you; GAO Ke; GAO Shi-juan; LI Yang-qiu

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Effect of cimetidine on clonal expansion of TCR Vβ subfamily of T cells in cord blood stimulated by K562 cells

更新时间：2025-01-15

- Effect of cimetidine on clonal expansion of TCR Vβ subfamily of T cells in cord blood stimulated by K562 cells
- Journal of Army Medical University Vol. 31, Issue 17, Pages: 1637-1640(2009)
- 作者机构：
  
  暨南大学医学院:微生物学与免疫学教研室,血液病研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R975;R392.12;R733.72
- Published：2009，
  
  Published Online：15 September 2009，
- 稿件说明：
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TIAN HONG-XIA, LIN CHEN, CHEN SHAO-HUA, et al. Effect of cimetidine on clonal expansion of TCR Vβ subfamily of T cells in cord blood stimulated by K562 cells. [J]. Journal of army medical university, 2009, 31(17): 1637-1640.
DOI：

TIAN HONG-XIA, LIN CHEN, CHEN SHAO-HUA, et al. Effect of cimetidine on clonal expansion of TCR Vβ subfamily of T cells in cord blood stimulated by K562 cells. [J]. Journal of army medical university, 2009, 31(17): 1637-1640. DOI：

摘要

目的研究甲氰咪呱（cimetidine）对K562细胞体外诱导脐血T细胞TCR Vβ亚家族克隆性的影响。方法体外分别将甲氰咪呱、K562细胞、甲氰咪呱+K562细胞与正常人的脐带血单个核细胞（mononuclear cells

MNC）混合培养2周，检测诱导增殖T细胞的特异杀伤活性，同时利用RT-PCR基因扫描技术分析培养后T细胞的TCR Vβ亚家族谱系的选择性利用和克隆性增殖情况。结果经甲氰咪呱、K562细胞、甲氰咪呱+ K562细胞诱导培养2周后，各组均不同程度诱导细胞增殖，其中甲氰咪呱联合K562细胞诱导组的T细胞对K562细胞的特异性杀伤作用显著增强（

0.01）。3组均呈现不同程度的T细胞TCR Vβ亚家族选择性利用，均有TCR Vβ21亚家族呈克隆性增殖。结论甲氰咪呱可协助增强bcr-abl抗原刺激的特异性抗CML T细胞作用。

Abstract

Objective To investigate the effect of cimetidine on the clonal expansion of TCR Vβ subfamily of T cells in cord blood after the stimulation by K562 cells

in vitro

. Methods Cimetidine (1×10

-5

mol/L) or K562 cells (1×10

/ml) or both of them were respectively cultured with mononuclear cells (MNC) isolated from normal human cord blood for 2 weeks. After the induction

specific cytotoxicity of the proliferated T cells were detected with K562 cells as the target cells. The selective usages and clonal expansion of TCR Vβ subfa-mily of T cells were analyzed by RT-PCR and genescan technique.

Results After induction for 2 weeks

the 3 groups showed the increased cell proliferation

in which specific cytotoxicity of T cells induced by both cimetidine and K562 cells against K562 cells was enhanced significantly compared with the other 2 groups (

0.01). Differental selective usages of T cells TCR Vβ subfamily and TCR Vβ21 clonal proliferation were found significantly in every group. Conclusion Cimetidine enhances the specific cytotoxicity of T cells stimulated by chronic myelogenous leukemia L antigen.

关键词

甲氰咪呱T细胞受体Vβ基因慢性粒细胞白血病脐血

Keywords

cimetidineT cell receptor Vβ genechronic myelogenous leukemiacord blood

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