Ultraviolet blood irradiation and oxygenation protects erythrocyte immune function in patients during cardiopulmonary bypass

ZHOU Tao; XIANG Dao-kang; ZHOU Liang-xian; SHU Yi-zhu; WU Xue-zhi; ZHANG Da-guo; YAN Xing-zhi; LIU Xiu-lun; LIU Xiao-bin

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Ultraviolet blood irradiation and oxygenation protects erythrocyte immune function in patients during cardiopulmonary bypass

更新时间：2025-01-15

- Ultraviolet blood irradiation and oxygenation protects erythrocyte immune function in patients during cardiopulmonary bypass
- Journal of Army Medical University Vol. 31, Issue 17, Pages: 1687-1689(2009)
- 作者机构：
  
  贵州省人民医院心脏外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R331.141;R454.2;R654.1
- Published：2009，
  
  Published Online：15 September 2009，
- 稿件说明：
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ZHOU TAO, XIANG DAO-KANG, ZHOU LIANG-XIAN, et al. Ultraviolet blood irradiation and oxygenation protects erythrocyte immune function in patients during cardiopulmonary bypass. [J]. Journal of army medical university, 2009, 31(17): 1687-1689.
DOI：

ZHOU TAO, XIANG DAO-KANG, ZHOU LIANG-XIAN, et al. Ultraviolet blood irradiation and oxygenation protects erythrocyte immune function in patients during cardiopulmonary bypass. [J]. Journal of army medical university, 2009, 31(17): 1687-1689. DOI：

摘要

目的研究紫外线照射充氧血对体外循环心内直视手术患者红细胞免疫黏附功能的影响。方法 67例行心脏瓣膜置换手术患者，使用随机数字表法分为治疗组（33例）和对照组（34例），治疗组于麻醉后通过锁骨下静脉按10 ml/kg 体质量放血或使用库血行紫外线照射充氧后加入预充液，对照组使用等量生理盐水，其他处理同治疗组。2组均于麻醉诱导后，转机30 min，术后第1、7天取外周血测定红细胞C3b受体花环率(RC3bRR

%)和红细胞免疫复合物花环率(RICRR，%)；2组均于升主动脉阻断前和开放时抽桡动脉血监测丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性。结果 2组RC3bRR、RICRR在转机30 min、术后第1、7天均低于麻醉诱导后，术后第7天尚未恢复至麻醉诱导后水平，但对照组较治疗组下降更明显（

0.05）；治疗组升主动脉开放时MDA含量低于对照组（

0.05），SOD活性则高于对照组（

0.05）。结论紫外线照射充氧血对体外循环手术患者红细胞免疫黏附功能有一定的保护作用，该保护作用可能通过维持红细胞自身结构完整和减轻自由基对红细胞的氧化损伤来实现。

Abstract

Objective To investigate the protective effect of ultraviolet blood irradiation and oxygenation (UBIO) on erythrocyte immune function in patients during cardiopulmonary bypass (CPB). Methods Sixty-seven patients undergoing elective heart valve replacements with CPB were randomly divided into control group (

=34) and experimental group (

=33). In experimental group

UBIO accumulated the blood from the subclavian vein (10 ml/kg) was dealt with ultraviolet i

rradiation (wave length 240 to 300 nm) and oxygenation (FiO

100%)

and then the ultraviolet-irradiated and oxygenated blood was added into cardiopulmonary-bypass circuit priming while equivalent quantitative saline was added to the control. Peripheral venous blood samples of all patients were collected at following time points

during and after cardiac surgery

after induction of anaesthesia

30 min after start of CPB

24 h and 7 d after surgery

which were anticoagulated with heparin for dynamically detecting the changes of RBC-C3bRR and RBC-ICR. The serum concentration of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were compared between the 2 groups before aortic cross-clamping and after aorta declamping. Results At the time of aorta declamping in experimental group

MDA was lower but SOD was higher than those of the control. RBC-C3bRR and RBC-ICR in the experimental group were significantly higher at 30 min after beginning of CPB

24 h

and 7 d after operation than those in the control (

0.05). Conclusion UBIO markedly mitigates the impairment of erythrocyte immune function caused by CPB and promotes the recovery of erythrocyte immunity in patients after CPB. This protective effect may be due to the maintenance of structural integrity of red blood cells and the attenuated injury of red blood cells by free radicals.

关键词

心肺转流术红细胞免疫紫外线照射充氧血

Keywords

cardiopulmonary bypasserythrocytesimmunityultraviolet blood irradiation and oxygenation

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