Clinical significance of MMP-12 expression in non-small cell lung cancer: report of 38 cases

Pan Hong; Liu Ming; Zhang Xiaoli; Zhang Xue; Huang Junfu; Wang Jue; Liu Chunjiang; Shuai Weizheng; Zhang Kejun; Fu Weiling

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Clinical significance of MMP-12 expression in non-small cell lung cancer: report of 38 cases

更新时间：2025-01-15

- Clinical significance of MMP-12 expression in non-small cell lung cancer: report of 38 cases
- Journal of Army Medical University Vol. 32, Issue 2, Pages: 142-145(2010)
- 作者机构：
  
  1. 第三军医大学西南医院：检验科,胸心外科
  2. 海军总医院ICU
  3. 第三军医大学大坪医院野战外科研究所检验科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394.2;R734.2;R730.23
- Published：2010，
  
  Published Online：30 January 2010，
- 稿件说明：
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PAN HONG, LIU MING, ZHANG XIAOLI, et al. Clinical significance of MMP-12 expression in non-small cell lung cancer: report of 38 cases. [J]. Journal of army medical university, 2010, 32(2): 142-145.
DOI：

PAN HONG, LIU MING, ZHANG XIAOLI, et al. Clinical significance of MMP-12 expression in non-small cell lung cancer: report of 38 cases. [J]. Journal of army medical university, 2010, 32(2): 142-145. DOI：

摘要

目的探讨非小细胞肺癌（non-small cell lung cancer，NSCLC）组织中MMP-12的表达及临床意义。方法应用逆转录-聚合酶链反应（RT-PCR）检测12例Ⅰ期和26例Ⅱ、Ⅲ期非小细胞肺癌及癌旁组织中MMP-12 mRNA的表达；Western blot检测部分标本中MMP-12蛋白表达。结合临床病理资料，比较MMP-12 mRNA表达与非小细胞肺癌临床病理特征的关系。结果 MMP-12 mRNA 在38例癌旁组织中无表达，在12例Ⅰ期和26例Ⅱ、Ⅲ期癌组织中呈不同程度的表达，半定量均值分别为（0.551±0.140）和（1.082±0.424），其差异有统计学意义（

=0.001）；MMP-12蛋白在癌旁组织中无表达，而在Ⅰ期癌组织中低表达，在Ⅱ、Ⅲ期癌组织中高表达。结论 MMP-12在非小细胞肺癌组织中高表达，并与淋巴结的转移存在显著相关性，表明MMP-12基因转录水平异常在非小细胞肺癌的发生、发展中起了重要作用。

Abstract

Objective To investigate the expression and clinical significance of matrix metalloproteinase-12 (MMP-12) in non-small cell lung cancer (NSCLC). Methods Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect MMP-12 mRNA expressions in 38 NSCLC the tumor tissues

including 12 samples of stage Ⅰ and 26 of stage Ⅱ and Ⅲ

and the matched normal lung tissues. Western blotting was used to measure the protein expression of MMP-12 in some tissue samples. Clinicopathological data were collected

and the relationship of MMP-12 mRNA and MMP-12 protein expression were analyzed with the clinicopathologi

cal characteristics of NSCLC. Results There was no expression of MMP-12 in 38 matched normal lung tissue

and MMP-12 mRNA was expressed in the NSCLC at stage Ⅰ (0.551±0.140)

and at stage Ⅱ and Ⅲ tumor tissues (1.082±0.424) with significant difference (

=0.001)

MMP-12 protein was not found in normal lung tissues

but expressed mild in the cancer tissue at stage Ⅰ

and highly in the tissues at stage Ⅱ and Ⅲ. Conclusion MMP-12 is highly expressed in NSCLC at stage of Ⅱ and Ⅲ

and significantly correlated with lymphatic node metastasis. The abnormal expression of MMP-12 in gene transcription may play an important role in the tumorogenesis and development of NSCLC.

关键词

非小细胞肺癌MMP-12逆转录-聚合酶链反应

Keywords

non-small cell lung cancermatrix metalloproteinase-12reverse transcription-polymerase chain reaction

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