Progesterone regulates COX-2 and Caspase-3 expressions in hippocampal neurons in rats after traumatic brain injury by NF-κB signaling pathway

Meng Jingshan; Si Daowen; Zhang Zuofeng; Zhang Yuxin; Zhang Ziming

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Progesterone regulates COX-2 and Caspase-3 expressions in hippocampal neurons in rats after traumatic brain injury by NF-κB signaling pathway

更新时间：2025-01-15

- Progesterone regulates COX-2 and Caspase-3 expressions in hippocampal neurons in rats after traumatic brain injury by NF-κB signaling pathway
- Journal of Army Medical University Vol. 32, Issue 2, Pages: 146-149(2010)
- 作者机构：
  
  华北煤炭医学院基础医学部解剖学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R651.15;R345;R977.12
- Published：2010，
  
  Published Online：30 January 2010，
- 稿件说明：
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MENG JINGSHAN, SI DAOWEN, ZHANG ZUOFENG, et al. Progesterone regulates COX-2 and Caspase-3 expressions in hippocampal neurons in rats after traumatic brain injury by NF-κB signaling pathway. [J]. Journal of army medical university, 2010, 32(2): 146-149.
DOI：

MENG JINGSHAN, SI DAOWEN, ZHANG ZUOFENG, et al. Progesterone regulates COX-2 and Caspase-3 expressions in hippocampal neurons in rats after traumatic brain injury by NF-κB signaling pathway. [J]. Journal of army medical university, 2010, 32(2): 146-149. DOI：

摘要

目的研究NF-κB对脑损伤大鼠海马神经元环氧合酶-2(COX-2)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的表达调控作用，探讨孕酮(progesterone，PROG)对脑损伤(TBI)后大鼠海马神经元保护作用及其可能机制。方法雄性SD大鼠45只按抛硬币法分为假手术组、损伤组和PROG组，按照改良的Feeney自由落体损伤装置制作大鼠脑损伤模型，用Nissl染色法、免疫组织化学法及免疫蛋白印记法，观察大鼠伤侧海马CA1区NF-κB、COX-2以及Caspase-3的表达变化和孕酮对上述指标变化的影响。结果损伤组大鼠海马CA1区NF-κB(24.0±2.5)、COX-2(35.9±2.7)、Caspase-3(25.1±2.7)阳性神经元的数量较假手术组NF-κB(1.9±0.9)、COX-2(1.5±0.7)、Caspase-3(1.8±0.8)显著增加(

0.05)；PROG组与损伤组比较，海马CA1区NF-κB(14.2±1.8)、COX-2(16.6±2.7)、Caspase-3(11.2±2.4)阳性神经元的数量明显减少(

0.05)。结论 NF-κB对脑损伤后大鼠海马神经元COX-2和Caspase-3表达起调控作用；PROG通过NF-κB对脑损伤后大鼠海马神经元起保护作用。

Abstract

Objective To investigate the effect of nuclear factor-κB (NF-κB) on the regulation of cyclooxygenase-2 (COX-2) and Caspase-3 in hippocampal neurons after traumatic brain injury (TBI) in rats

and explore the neuroprotective effect and the possible mechanism of progesterone (PROG) in hippocampal neurons after TBI. Methods Forty-five male Spraque-Dawley rats were randomly divided into 3 groups: sham-ope

rated group (

=15)

TBI group (

=15) and PROG-treated group (

=15

intraperitoneal injection of PROG 16 mg/kg in 1 and 6 h after injury). The rat model of TBI was duplicated with the improved Feeney’s method. The rats were sacrificed in 24 h after injury and their brain was resected. Nissl staining

immunohistochemical staining and Western blot assay for NF-κB

COX-2 and Caspase-3 was used to observe the changes of positive cell numbers and protein levels in the hippocampal neurons. Results The numbers of immunoreactive neurons to NF-κB (24.0±2.5)

COX-2 (35.9±2.7) and Caspase-3 (25.1±2.7) were significantly increased in the hippocampus at 24 h after TBI when compared with the positive neuron numbers of NF-κB (1.9±0.9)

COX-2 (1.5±0.7) and Caspase-3 (1.8±0.8) in sham group. After the treatment of PROG

the positive cell number of NF-κB (14.2±1.8)

COX-2 (16.6±2.7)

Caspase-3 (11.2±2.4) was reduced obviously as compared with the TBI group (

0.05). Conclusion NF-κB signaling pathway may play an important role in the regulation of COX-2 and Caspase-3 in the hippocampus neurons after TBI in rats. PROG may influence the expression of NF-κB and protect the hippocampal neurons.

关键词

脑损伤孕酮核因子-κB环氧合酶-2半胱氨酸天冬氨酸蛋白酶-3

Keywords

traumatic brain injuryprogesteronenuclear factor-κBcyclooxygenase-2Caspase-3

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