Expression change of P2X<sub>3</sub> receptor in spinal dorsal horn and dorsal root ganglion in inflammatory pain rats

Du Lu; Zhang Jinhai; Ruan Huaizhen

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Expression change of P2X₃ receptor in spinal dorsal horn and dorsal root ganglion in inflammatory pain rats

更新时间：2025-01-15

- Expression change of P2X₃ receptor in spinal dorsal horn and dorsal root ganglion in inflammatory pain rats
- Journal of Army Medical University Vol. 32, Issue 2, Pages: 99-102(2010)
- 作者机构：
  
  第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.25;R441.1;R322.81
- Published：2010，
  
  Published Online：30 January 2010，
- 稿件说明：
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DU LU, ZHANG JINHAI, RUAN HUAIZHEN. Expression change of P2X₃ receptor in spinal dorsal horn and dorsal root ganglion in inflammatory pain rats. [J]. Journal of army medical university, 2010, 32(2): 99-102.
DOI：

DU LU, ZHANG JINHAI, RUAN HUAIZHEN. Expression change of P2X₃ receptor in spinal dorsal horn and dorsal root ganglion in inflammatory pain rats. [J]. Journal of army medical university, 2010, 32(2): 99-102. DOI：

摘要

目的观察足底皮下注射角叉菜胶致炎后大鼠行为学及脊髓背角和背根神经节（dorsal root ganglion

DRG）P2X

受体表达的变化。方法通过热辐射刺激法及von Frey纤维丝测定大鼠痛阈，应用免疫组化技术观察足底皮下注射角叉菜胶后不同时间其相应节段（L

～L

）脊髓及背根神经节P2X

受体表达的变化。结果足底皮下注射角叉菜胶后动物出现热痛敏和机械性痛敏，热痛阈和机械痛阈均下降，于12 h达最低，72 h后恢复至正常水平。免疫组化显示相应节段（L

～L

）脊髓背角P2X

受体免疫反应在注射角叉菜胶24 h后明显增强（

0.01），72 h表达强度最高，168 h恢复至正常，并且相应节段背根神经节P2X

受体阳性神经元数12 h开始明显增加（

0.01），持续至72 h，168 h恢复至正常。结论角叉菜胶所致炎性痛大鼠，脊髓背角和背根神经节P2X

受体活化，表达明显上调，表明P2X

受体在炎性痛的发生、发展中起重要作用。

Abstract

Objective To investigate the expression change of P2X

receptor in spinal dorsal horn and dorsal root ganglion (DRG) in rats with experimental inflammatory pain. Methods Totally 30 SD rats received a subcutaneous injection of carrageenan in the right paw to establish inflammatory pain model

and then divided randomly and equally into 5 groups

that is 6

72 and 168 h after injection. Another 6 rats undergoing a subcutaneous injectio

n of normal saline in the right paw served as control. Pain threshold was assayed by thermal radiation and von Frey filament in 2

72 and 168 h after injection. Expression of P2X

receptor in spinal dorsal horn and DRG of every group was measured by immunohistochemical staining (IHC). Results After injection of carrageenan

thermal hyperalgesia and mechanical allodynia appeared in the rats. The thermal withdrawl latency (TWL) and 50% paw withdrawl threshold (PWT) were decreased obviously from 2 h

descended to the lowest at 12 h

then returned to the normal level after 72 h. The expression of P2X

receptor in spinal dorsal horn was increased obviously from 24 h，while that at DRG was increased obviously from 12 h. Both of the expressions lasted for 72 h

and returned to the normal level at 168 h after injection. Conclusion Thermal hyperalgesia and mechanical allodynia appear in the rats after carrageenan injection

and the P2X

receptor is activated and up-regulated in the DRG and spinal cord

suggesting that P2X

receptor may play an important role in the incidence and development of inflammatory pain.

关键词

P2X3受体背根神经节脊髓角叉菜胶疼痛

Keywords

P2X3 receptordorsal root ganglionspinal cordcarrageenanpain

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Expression change of P2X3 receptor in spinal dorsal horn and dorsal root ganglion in inflammatory pain rats

DOI：

摘要

Abstract

关键词

Keywords

references

Expression change of P2X₃ receptor in spinal dorsal horn and dorsal root ganglion in inflammatory pain rats