Molecular epidemiology and drug resistant mechanisms of carbapenem-resistant <em>Acinetobacter baumannii</em>

Zhang Xiaobing; Liu Xing; Gong Yali; Fu Weiling

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Molecular epidemiology and drug resistant mechanisms of carbapenem-resistant Acinetobacter baumannii

更新时间：2025-01-15

- Molecular epidemiology and drug resistant mechanisms of carbapenem-resistant Acinetobacter baumannii
- Journal of Army Medical University Vol. 33, Issue 5, Pages: 502-505(2011)
- 作者机构：
  
  第三军医大学西南医院检验科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R181.32;R978.11;R378
- Published：2011，
  
  Published Online：15 March 2011，
- 稿件说明：
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ZHANG XIAOBING, LIU XING, GONG YALI, et al. Molecular epidemiology and drug resistant mechanisms of carbapenem-resistant Acinetobacter baumannii. [J]. Journal of army medical university, 2011, 33(5): 502-505.
DOI：

ZHANG XIAOBING, LIU XING, GONG YALI, et al. Molecular epidemiology and drug resistant mechanisms of carbapenem-resistant Acinetobacter baumannii. [J]. Journal of army medical university, 2011, 33(5): 502-505. DOI：

摘要

目的分析我院碳青霉烯类耐药的鲍曼不动杆菌分子流行病学及耐药机制。方法鲍曼不动杆菌源自2008年1-12月我院临床分离株，共51株。采用K-B法（CLSI 2008年标准）筛选对碳青霉烯类耐药的鲍曼不动杆菌临床分离株，采用随机引物多态性DNA(randomly amplified polymorphism DNA， RAPD)分型对其进行分子流行病学研究；PCR方法检测TEM、SHV、CTX-M、IMP-1、IMP-2、VIM、VIM-2、OXA-23、OXA-24等耐药基因型以及Ⅰ、Ⅱ类整合酶。结果 51株碳青霉烯类耐药鲍曼不动杆菌为多重耐药菌株，除阿米卡星外，其他抗生素耐药率数据非常高；其中50株为同一克隆株；TEM、SHV、CTX-M、IMP-1、VIM-2、OXA-23、Int1基因阳性率分别为98.0%、23.5%、19.6%、25.5%、19.6%、72.5%、86.3%；而IMP-2、VIM、OXA-24和Int-2基因均为阴性。结论我院碳氢霉烯类耐药鲍曼不动杆菌暴发流行，携带TEM型β-内酰胺酶、OXA-23、IMP-1和VIM-2型碳青霉烯酶基因和Ⅰ类整合酶。产生OXA-23型碳青霉烯酶和金属酶是我院鲍曼不动杆菌对碳氢霉烯类耐药的主要机制。

Abstract

Objective To investigate the molecular epidemiology and drug resistance mechanism of carbapenem-resistant

Acinetobacter baumannii

(CRAB). Methods Antimicrobial susceptibility was tested on 51 strains of

Acinetobacter baumannii

isolated in our hospital from January to December 2008 by Kirby-Bauer method. The results were assessed according to the standards recommended by CLSI (2008). The homology of 51 isolates of CRAB was determined by randomly amplifie

d polymorphism DNA (RAPD). The genotype of TEM

SHV

CTX-M

IMP-1

IMP-2

VIM

VIM-2

OXA-23

OXA-24 and class Ⅰ

Ⅱ integrase (Int-1 and Int-2) was detected by polymerase chain reaction (PCR). Results These 51 strains

Acinetobacter baumannii

were all multi-drug resistant

and were resistant to most antibiotics except amikacin. Among them

50 strains were the same clone. Positive rate of these strains to TEM

SHV

CTX-M

IMP-1

VIM-2

OXA-23 and Int1 was 98.0%

23.5%

19.6%

25.5%

19.6%

72.5%

and 86.3%

respectively. But no genotype of IMP-2

VIM

OXA-24 and Int-2 was detected. Conclusion The stain of CRAB with TEM

OXA-23

IMP-1

VIM-2 and Int-2 genotypes breaks out in our hospital. Its production of OXA-23 carbapenemase and metallo-β-lactamase might be the main mechanisms of carbapenem resistance .

关键词

鲍曼不动杆菌碳青霉烯类耐药性

Keywords

Acinetobacter baumanniicarbapenemsresistance

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⁰

Molecular epidemiology and drug resistant mechanisms of carbapenem-resistant Acinetobacter baumannii

DOI：

摘要

Abstract

关键词

Keywords

references