Expression of matrix metalloproteinase 8 in esophageal squamous cell carcinoma

Wang Dongmei; Guo  Hong; Chen Xuedan; Guan Xingying; Liu  Dan; Bai  Yun

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Expression of matrix metalloproteinase 8 in esophageal squamous cell carcinoma

更新时间：2025-01-15

- Expression of matrix metalloproteinase 8 in esophageal squamous cell carcinoma
- Journal of Army Medical University Vol. 33, Issue 5, Pages: 519-522(2011)
- 作者机构：
  
  1. 第三军医大学基础医学部医学遗传学教研室
  2. 解放军161医院检验科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394.3;R730.2;R735.1
- Published：2011，
  
  Published Online：15 March 2011，
- 稿件说明：
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WANG DONGMEI, GUO HONG, CHEN XUEDAN, et al. Expression of matrix metalloproteinase 8 in esophageal squamous cell carcinoma. [J]. Journal of army medical university, 2011, 33(5): 519-522.
DOI：

WANG DONGMEI, GUO HONG, CHEN XUEDAN, et al. Expression of matrix metalloproteinase 8 in esophageal squamous cell carcinoma. [J]. Journal of army medical university, 2011, 33(5): 519-522. DOI：

摘要

目的探讨食管鳞状细胞癌中基质金属蛋白酶-8（matrix metalloproteinase 8

MMP-8）的表达及其与肿瘤浸润、转移的关系。方法实时荧光定量PCR方法检测67例食管鳞癌组织和27例远癌组织中MMP-8 mRNA 的表达；Western blot法检测其中15例食管鳞癌组织及其配对的15例远癌组织中MMP-8蛋白的表达，统计分析MMP-8表达水平与食管鳞癌的临床分期以及浸润转移的关系。结果 ①MMP-8在食管鳞癌组织和远癌组织中均有不同程度的表达，在癌组织的表达水平显著低于远癌组织（mRNA表达水平

=0.003，蛋白表达水平

=0.031）。②T1、T2、T3、T4期食管鳞癌组织中MMP-8 mRNA表达存在显著差异（

=0.002），MMP-8表达随着肿瘤浸润程度的加深而升高；MMP-8蛋白表达量在T1～T4期中也呈增加的趋势。③MMP-8 mRNA和蛋白表达水平在食管鳞癌的不同淋巴结转移组之间没有显著差异。结论 MMP-8在食管鳞癌组织中低表达，但又随肿瘤浸润加深而表达升高，可能反映了MMP-8在食管鳞癌发生和浸润中的不同作用。

Abstract

Objective To study the expression of matrix metalloproteinase 8 (MMP-8) in esophageal squamous cell carcinoma (ESCC) and its relationship with tumor invasion and metastasis. Methods MMP-8 mRNA expressions in 67 ESCC tissue specimens and in 27 normal tissue specimens were detected by real-time PCR. MMP-8 protein expression in 15 paired specimens of ESCC and in normal tissues were detected by Western blotting. The relationship between MMP-8 expression level and the clinical stage

invasion and metastasis of ESCC was statistic

ally analyzed. Results MMP-8 expression was detected in both ESCC tissues and normal tissues

and the expression level in ESCC tissues was significantly lower than that in normal tissues (

=0.003 for mRNA expression and

=0.031 for protein expression). MMP-8 expression in ESCC tissues had significant differences among T1

T3 and T4 stages (

=0.002)

increasing from T1 to T4 stage

and rose when tumor invasion degree heightened. No obvious correlation was found between the MMP-8 mRNA and protein expression levels and lymph node metastasis of ESCC. Conclusions Expression of MMP-8 is low in ESCC tissues and increases with elevated invasion degree

which may reflect different mechanisms of MMP-8 in ESCC carcinogenesis and invasion.

关键词

食管鳞状细胞癌基质金属蛋白酶8浸润转移表达

Keywords

esophageal squamous cell carcinomasmatrix metalloproteinaseinvasionmetastasisexpression

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