Demineralized bone matrix combined with self-assembling peptide as scaffold for enriching nucleated cells in bone marrow

Li Zhiqiang; Hou Tianyong; Luo Fei; Xu Jianzhong

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Demineralized bone matrix combined with self-assembling peptide as scaffold for enriching nucleated cells in bone marrow

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- Demineralized bone matrix combined with self-assembling peptide as scaffold for enriching nucleated cells in bone marrow
- Journal of Army Medical University Vol. 33, Issue 5, Pages: 441-444(2011)
- 作者机构：
  
  第三军医大学西南医院骨科, 全军矫形外科中心
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.71;R329.24;R318.08
- Published：2011，
  
  Published Online：15 March 2011，
- 稿件说明：
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LI ZHIQIANG, HOU TIANYONG, LUO FEI, et al. Demineralized bone matrix combined with self-assembling peptide as scaffold for enriching nucleated cells in bone marrow. [J]. Journal of army medical university, 2011, 33(5): 441-444.
DOI：

LI ZHIQIANG, HOU TIANYONG, LUO FEI, et al. Demineralized bone matrix combined with self-assembling peptide as scaffold for enriching nucleated cells in bone marrow. [J]. Journal of army medical university, 2011, 33(5): 441-444. DOI：

摘要

目的观察纳米自组装肽RADA16-Ⅰ与脱钙骨基质复合富集材料的表征及性能，为选择性细胞滞留技术（selective cell retention

SCR)提供一种优化的骨髓富集材料。方法 RADA16-Ⅰ在阳离子触发下与脱钙骨基质（demineralized bone matrix

DBM）构建复合材料。三维视频显微镜检测孔径大小，X线光电子能谱分析仪（XPS）检测RADA16-Ⅰ与DBM的结合情况，SCR技术检测复合材料对骨髓中有核细胞的富集效果，切片经HE染色后，光学显微镜下观察组织结构。结果 RADA16-Ⅰ水凝胶可有效黏附于DBM材料；RADA16-Ⅰ/DBM的孔径(220.55±158.54)μm显著小于DBM 孔径（414.32±175.00)μm（

0.05）；RADA16-Ⅰ/DBM对骨髓中有核细胞的富集倍数为（6.05±0.96）倍显著高于DBM的（2.27±0.41）倍（

0.05）。结论 RADA16-Ⅰ/DBM复合富集材料能够明显增强骨髓中有核细胞的黏附，可作为一种有效的骨修复材料。

Abstract

Objective To investigate the characteristics and performance of demineralized bone matrix (DBM) combined with nanoscale self-assembling peptide RADA16-Ⅰ (RADA16-Ⅰ/DBM)

and to evaluate its application as an optimal scaffold for enriching nucleated cells in bone marrow in selective cell retention (SCR). Methods DBM was combined with RADA16-Ⅰ by a cation-triggered process. The pore sizes of RADA16-Ⅰ/DBM were measured using a 3D video microscope. The combination of RADA16-Ⅰ and DBM was examined by X-

ray photoelectron spectroscopy (XPS). The performances of RADA16-Ⅰ/DBM and DBM in enriching nucleated cells in bone marrow were tested by SCR. The structures of RADA16-Ⅰ/DBM and DBM that had enriched nucleated cells were observed under a microscope after HE staining. Results RADA16-Ⅰ/DBM had significantly smaller pore sizes than DMB (220.55±158.54 μm

414.32±175.00 μm

0.05). RADA16-Ⅰ hydrogel could firmly adhere to DBM. RADA16-Ⅰ/DBM had a significantly higher enrichment ratio than DMB for nucleated cells in bone marrow (6.05±0.96

2.27±0.41

0.05). Conclusion RADA16-Ⅰ/DBM possesses improved performance in enriching nucleated cells in bone marrow

and can be used as an effective bone-repair material.

关键词

自组装肽脱钙骨基质有核细胞富集材料

Keywords

self-assembling peptidesdemineralized bone matrixnucleated cellenrichment materials

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