Objective    To explore the effect of krüppel-like factor 4 (KLF4) on

in-vitro

proliferation of human stomach cancer cell line SGC-7901.     Methods    SGC-7901 cells were subjected to liposome-mediated transfection with recombinant plasmid pcDNA3.1IE-KLF4 to establish a recombinant cell line for stable over-expression of KLF4. Meanwhile

untransfected SGC-7901 cells and SGC-7901 cells transfected with empty plasmid vector were used as controls. RT-PCR and immunocytochemical assay were employed to test mRNA and protein express

ion of KLF4

and flow cytometry was employed to detect the cell cycle and proliferation index.     Results    RT-PCR and immunocytochemical assay results showed that mRNA and protein expression of KLF4 was significant in the recombinant cell line as compared with the controls (

P

<

0.05). Flow cytometry results demonstrated that the recombinant cells in G

1

phase increased [(65.08±3.43)%

P

<

0.05

]

while those in S phase and G

2

/M phase decreased [(30.39±1.80)% and (4.53±1.66)%

P

<

0.05

]

and the proliferation index of the recombinant cells also decreased [(34.92±3.42)%

P

<

0.05

]

.     Conclusion    KLF4 inhibits the

in-vitro

proliferation of human stomach cancer cell line SGC-7901.