Objective    To investigate the mutation of telomerase inhibitor Pin2/TRF1-interacting protein X1 (PinX1) gene in cervical carcinoma cell lines

healthy cervical tissues

and cervical carcinoma tissue specimens.     Methods    Three cervical carcinoma cell lines HeLa

C-33A

and CaSki (lymphoma cell lines Namalwa

Raji

and Daudi

and breast cancer cell line MCF-7 as controls)

twenty-eight healthy cervical tissues

and fifty-two cervical carcinoma tissue specimens were selected for PinX1 gene mutation analysis. With genome DNA extracted from the above cells and specimens as

templates

seven extrons and part flanking sequences of PinX1 gene were amplified through PCR. Purified PCR products were sequenced directly to detect PinX1 gene mutation. The data were analyzed with SPSS.     Results    Three mutation sites were detected in cervical carcinoma cell lines. Five mutation sites were detected in cervical carcinoma tissues

among which Ser

254

Cys in extron 7 and IVS2+18 G→A in intron 2 had mutation rates of 23.1% and 51.9%

respectively

significantly different from those of healthy cervical tissues (

P

<

0.05). Mutation site IVS2+64 C→A (mutation rate=9.3%) in intron 2 was first reported.     Conclusion    Unknown mutation sites of PinX1 gene may exist in cervical carcinoma. The study about PinX1 gene mutation may reveal new targets for diagnosis

prevention

and personalized treatment of cervical carcinoma.