Effect of cholecystokinin octapeptide on IL-1β, IL-6 and IL-10 expression in LPS-induced RAW 264.7 cells

Ni Zhiyu; Cong Bin; Dong Mei; Ma Chunling; Li Shujin; Wen Di; Xu Zhilu

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Effect of cholecystokinin octapeptide on IL-1β, IL-6 and IL-10 expression in LPS-induced RAW 264.7 cells

更新时间：2025-01-15

- Effect of cholecystokinin octapeptide on IL-1β, IL-6 and IL-10 expression in LPS-induced RAW 264.7 cells
- Journal of Army Medical University Vol. 33, Issue 22, Pages: 2366-2369(2011)
- 作者机构：
  
  河北医科大学基础医学院法医学系，河北省法医学重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392-33;R392.12;R392.32
- Published：2011，
  
  Published Online：30 November 2011，
- 稿件说明：
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NI ZHIYU, CONG BIN, DONG MEI, et al. Effect of cholecystokinin octapeptide on IL-1β, IL-6 and IL-10 expression in LPS-induced RAW 264.7 cells. [J]. Journal of army medical university, 2011, 33(22): 2366-2369.
DOI：

NI ZHIYU, CONG BIN, DONG MEI, et al. Effect of cholecystokinin octapeptide on IL-1β, IL-6 and IL-10 expression in LPS-induced RAW 264.7 cells. [J]. Journal of army medical university, 2011, 33(22): 2366-2369. DOI：

摘要

目的研究脂多糖（lipopolysaccharide，LPS）诱导RAW 264.7细胞促炎性细胞因子IL-1β、IL-6及抗炎性细胞因子IL-10的动态变化规律及八肽胆囊收缩素（cholecystokinin octapeptide

CCK-8）对其表达的影响。方法 LPS诱导RAW 264.7细胞不同时间（0、3、6、12、24、48 h），在各细胞因子表达高峰的时间点用ELISA及RT-PCR法检测不同剂量（10

-10

、10

-9

、10

-8

、10

-7

、10

-6

mol/L）CCK-8对LPS诱导RAW 264.7细胞IL-1β、IL-6、IL-10蛋白及mRNA表达的影响。结果 ①LPS可诱导RAW 264.7细胞IL-1β、IL-6、IL-10蛋白合成及mRNA表达增加(

0.05

0.01)：各细胞因子蛋白的浓度分别于LPS刺激后6、24、24 h达到高峰[分别为（209.41±10.04）、（2 071.64±9.08）、（207.65±16.27）ng/L

]

；各细胞因子mRNA的表达分别于LPS刺激后3、24、24 h达到高峰[分别为（0.723±0.030）、（1.157±0.045）、(0.767±0.035)

]

。②CCK-8可剂量依赖性抑制IL-1β、IL-6的表达，并使IL-10表达进一步增加(

0.01)，且孵育细胞24 h，CCK-8上调LPS诱导IL-10生成的幅度要大于孵育48 h。结论 CCK-8通过抑制LPS诱导RAW 264.7细胞抑制促炎性细胞因子IL-1β、IL-6的表达和进一步增加抗炎性细胞因子IL-10的表达参与抗炎反应过程。

Abstract

Objective To observe the dynamic changes of proinflammatory cytokines IL-1β

IL-6 and anti-inflammatory cytokines IL-10 in LPS-induced RAW 264.7 cells and observe the effects of cholecystokinin o

ctapeptide (CCK-8) on their expression. Methods After RAW 264.7 cells were stimulated with 1 mg/L LPS for 0

24 and 48 h respectively

the expression peak times of IL-1β

IL-6 and IL-10 were selected to investigate the effects of CCK-8 at the doses of 10

-10

-9

-8

-7

and 10

-6

mol/L respectively. Cytokines protein and mRNA expressions were assayed by ELISA and RT-PCR. Results The expression of IL-1β

IL-6 and IL-10 mRNA and protein were induced by LPS in RAW 264.7 cells in a time-dependent manner. CCK-8 inhibited LPS-induced IL-1β and IL-6 expression and augmented IL-10 expression in a dose-dependent manner. When the cells were treated for 24 h

CCK-8 augmentation resulted in higher LPS-induced IL-10 expression when compared with that after 48 hours’ treatment. Conclusion CCK-8 may participate in the anti-inflammatory response by inhibiting the expression of proinflammatory cytokines IL-1β and IL-6

meanwhile augmenting the expression of anti-inflammatory cytokine IL-10 induced by LPS.

关键词

胆囊收缩素脂多糖RAW 264.7细胞IL-1βIL-6IL-10

Keywords

cholecystokininlipopolysaccharidesRAW 264.7 cellIL-1βIL-6IL-10

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