Citicoline pretreatment protects rats against spinal cord ischemia-reperfusion injury

Zeng Xiaohua; Liu Danyan; An Min

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Citicoline pretreatment protects rats against spinal cord ischemia-reperfusion injury

更新时间：2025-01-15

- Citicoline pretreatment protects rats against spinal cord ischemia-reperfusion injury
- Journal of Army Medical University Vol. 33, Issue 24, Pages: 2587-2591(2011)
- 作者机构：
  
  重庆医科大学附属第一医院麻醉科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R364.12;R965;R654.1
- Published：2011，
  
  Published Online：30 December 2011，
- 稿件说明：
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ZENG XIAOHUA, LIU DANYAN, AN MIN. Citicoline pretreatment protects rats against spinal cord ischemia-reperfusion injury. [J]. Journal of army medical university, 2011, 33(24): 2587-2591.
DOI：

ZENG XIAOHUA, LIU DANYAN, AN MIN. Citicoline pretreatment protects rats against spinal cord ischemia-reperfusion injury. [J]. Journal of army medical university, 2011, 33(24): 2587-2591. DOI：

摘要

目的探讨胞二磷胆碱对脊髓缺血再灌注损伤的影响及其作用机制。方法雄性SD大鼠72只，体质量（230±20）g，按随机数字表法分为3组（

=24）：假手术组，缺血再灌注损伤组（IR组）和胞二磷胆碱预先给药组（citi组），各组按灌注后12、24、48、72 h分成4个亚组（

=6）。各组均采用改良Zivin法建立脊髓缺血再灌注损伤模型，并于灌注后12、24、48、72 h 4个时点采用改良Tarlov法对大鼠脊髓运动和感觉功能进行评分，HE染色观察脊髓L

～L

节段的病理学改变，TUNEL法检测脊髓凋亡细胞数，于再灌注后72 h处采用免疫组化方法检测脊髓神经元Bcl-2、Bax、caspase-9表达及Bcl-2与Bax的比值（Bcl-2/Bax）。结果与假手术组相比，IR组和citi组运动和感觉评分均降低，HE染色有明显的病理损伤，TUNEL染色神经细胞凋亡数量增加，免疫组化显示caspase-9 、Bcl-2、Bax蛋白表达增加，Bcl-2/Bax降低（

0.01）；与IR组相比，citi组运动和感觉评分高，HE染色形态学损伤较轻，TUNEL染色神经细胞凋亡数量少，免疫组化Bcl-2表达明显增加，Bax、caspase-9表达降低，Bcl-2/Bax升高（

0.01）。结论胞二磷胆碱可减轻大鼠脊髓缺血再灌注损伤，改善神经功能，其机制可能与其上调Bcl-2蛋白表达，下调Bax、caspase-9蛋白表达，从而减轻神经元凋亡有关。

Abstract

Objective To determine the effects of citicoline pretreatment on spinal cord ischemia-reperfusion (IR) injury in rats. Methods Seventy-two male SD rats weighing 210 to 250 g were randomly divided into 3 groups (

=24): sham operation group

ischemia-reperfusion injury group (group IR) and citicoline pretreatment group

(group citi). Every group was divided into 4 subgroups according to the time after reperfusion for 12

48 and 72 h respectively (

=6). After modified Zivin methods was used to establish model

Tarlov with modification was used for neurological function scores at the above mentioned time points

HE staining was employed for L

2-5

spinal cord pathological changes

TUNEL method for calculation apoptosis cells

and immunohistochemical assay for Bcl-2

Bax

caspase-9 expression. The ratio of Bcl-2 to Bax (Bcl-2/Bax) was calculated. Results Compared to Sham group

group IR and group citi showed significantly lower neurological function scores

obvious pathological injury

increased apoptotic nerve cells

enhanced expression of caspase-9

Bax and Bcl-2

and significantly lower Bcl-2/Bax ratio（

0.01）. However

citi pretreatment resulted in better outcomes in above items compared to group IR（

0.01）. Conclusion Citicoline pretreatment attenuates spinal cord IR injury

improves neurological function through up-regulating Bcl-2

down-regulating Bax and caspase-9

and thus reducing neuronal apoptosis.

关键词

脊髓胞二磷胆碱预处理缺血再灌注损伤凋亡

Keywords

spinal cordciticolinepretreatmentischemia-reperfusionapoptosis

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