Changes of hydrogen sulfide/cystathionine-β-synthase system in hippocampus of heroin-addictive rats

Luo Xiaomei; Chen Yuanshou; Pan Guishu; Jin Huan

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Changes of hydrogen sulfide/cystathionine-β-synthase system in hippocampus of heroin-addictive rats

更新时间：2025-01-15

- Changes of hydrogen sulfide/cystathionine-β-synthase system in hippocampus of heroin-addictive rats
- Journal of Army Medical University Vol. 33, Issue 20, Pages: 2177-2179(2011)
- 作者机构：
  
  遵义医学院生理学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R749.61;R341;R363.27
- Published：2011，
  
  Published Online：30 October 2011，
- 稿件说明：
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LUO XIAOMEI, CHEN YUANSHOU, PAN GUISHU, et al. Changes of hydrogen sulfide/cystathionine-β-synthase system in hippocampus of heroin-addictive rats. [J]. Journal of army medical university, 2011, 33(20): 2177-2179.
DOI：

LUO XIAOMEI, CHEN YUANSHOU, PAN GUISHU, et al. Changes of hydrogen sulfide/cystathionine-β-synthase system in hippocampus of heroin-addictive rats. [J]. Journal of army medical university, 2011, 33(20): 2177-2179. DOI：

摘要

目的观察海洛因成瘾对大鼠硫化氢(hydrogen sulfide

S)/胱硫醚β-合酶(cystathionine-β-synthase

CBS)体系产生的影响。方法实验大鼠分成健康对照组、heroin组、heroin+硫氢化钠（NaHS）组

每组10只。 heroin组、heroin+NaHS组按剂量递增原则皮下注射海洛因9 d，对照组按同样方式注射等量生理盐水，heroin+NaHS组在每次注射海洛因前30 min腹腔注射NaHS，第10天纳洛酮腹腔注射观察戒断症状。采用亚甲基蓝比色法测定大鼠血浆及海马组织H

S含量，Real-time PCR检测海马组织CBS mRNA表达量。结果 heroin组与健康对照组比较

血浆及海马H

S含量均明显降低(

0.05)，CBS mRNA表达量显著增高(

0.05)；heroin+NaHS组与健康对照组比较，海马H

S含量明显降低(

0.05)；heroin+NaHS组与heroin组比较，血浆及海马H

S含量明显增高(

0.05)，CBS mRNA表达量明显降低(

0.05)。结论海洛因依赖可导致大鼠体内H

S水平下降，内源性H

S与CBS之间存在负反馈调节作用，使内源性H

S维持在生理浓度水平。

Abstract

Objective To determine the effect of heroin addiction on hydrogen sulfide (H

S)/cystathionine-β-synthase (CBS) system in the hippocampus of rats. Methods Experimental rats were randomly divided into 3 groups

control group

heroin group and heroin+NaHS group. The rats of 2 later groups received a subc

utaneous injection of heroin at a decreasing dose for 9 d and peritoneal injection of normal saline or NaHS (0.09 mmol/kg) in 30 min before heroin injection. Withdrawal symptoms on the 10th day were used to evaluate the model establishment. The plasma and hippocampal level of H

S were detected by the blue colorimetric method. The expression of CBS mRNA was observed by real time PCR. Results Compared with control group

S in plasma and hippocampus was significantly decreased (

0.05) while the expression of CBS mRNA was up-regulated (

0.05) in heroin group

but hippocampal level of H

S was decreased in heroin+NaHS group (

0.05). Compared with heroin group

S in plasma and hippocampus were elevated (

0.05)

and the expression of CBS mRNA was down-regulated in heroin+NaHS group (

0.05). Conclusion Heroin-addiction can decrease H

S level and up-regulate the expression of CBS mRNA in the hippocampus of rats.

关键词

海洛因依赖海马硫化氢胱硫醚β-合酶大鼠

Keywords

heroin dependencehippocampushydrogen sulfidecystathionine-β-synthaserats

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