Correlation between inhibitory effect of resveratrol on proliferation of A549 human lung andenocarcinoma cells and IGF-ⅠR

Zhu Jianwu; Xia Lei; Yang Jian; Luo Wei; Li Juan; Zhou Qian; Zeng Linli; Wang Dong; Yang Zhenzhou

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Correlation between inhibitory effect of resveratrol on proliferation of A549 human lung andenocarcinoma cells and IGF-ⅠR

更新时间：2025-01-15

- Correlation between inhibitory effect of resveratrol on proliferation of A549 human lung andenocarcinoma cells and IGF-ⅠR
- Journal of Army Medical University Vol. 33, Issue 24, Pages: 2567-2570(2011)
- 作者机构：
  
  第三军医大学大坪医院野战外科研究所：肿瘤中心，营养科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R979.19;R734.2;R730.23
- Published：2011，
  
  Published Online：30 December 2011，
- 稿件说明：
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ZHU JIANWU, XIA LEI, YANG JIAN, et al. Correlation between inhibitory effect of resveratrol on proliferation of A549 human lung andenocarcinoma cells and IGF-ⅠR. [J]. Journal of army medical university, 2011, 33(24): 2567-2570.
DOI：

ZHU JIANWU, XIA LEI, YANG JIAN, et al. Correlation between inhibitory effect of resveratrol on proliferation of A549 human lung andenocarcinoma cells and IGF-ⅠR. [J]. Journal of army medical university, 2011, 33(24): 2567-2570. DOI：

摘要

目的探讨白藜芦醇对人肺腺癌A549细胞增殖的抑制效应及其与IGF-ⅠR的关系。方法以人肺腺癌A549细胞株为研究对象，采用MTT方法测定细胞增殖，用RT-PCR与免疫印迹方法测定IGF-Ⅰ受体mRNA与蛋白表达水平。结果 2.5～15 nmol/L IGF-Ⅰ可显著促进A549细胞增殖（

0.05），给予12.5、25.0、50 μmol/L白藜芦醇对A549细胞增殖具有明显抑制作用（

0.05）。白藜芦醇（6.25～50 μmol/L）与10 nmol/L IGF-Ⅰ共作用于A549细胞，均可抑制IGF-Ⅰ的促A549细胞增殖作用，其中尤以25、50 μmol/L白藜芦醇的抑制增殖作用显著（

0.05），白藜芦醇可有效降低IGF-ⅠR mRNA与蛋白表达。结论白藜芦醇可通过降低IGF-ⅠR mRNA与蛋白表达有效抑制IGF-Ⅰ介导的A549肺癌细胞增殖。

Abstract

Objective To study the correlation between inhibitory effect of resveratrol on proliferation of A549 human lung adenocarcinoma cells and IGF-ⅠR. Methods Proliferation of A549 human lung andenocarcinoma cells was detected by methyl thiazolyl tetrazolium (MTT) assay. Expression level of IGF-ⅠR mRNA and protein was measured by RT-PCR and Western blotting

respectively. Results IGF-Ⅰ at the concentration of 2.5 to 15 nmol/L significantly increased the proliferation of A549 human lung andenocarcinoma cells (

0.05) and resvera

trol at the concentrations of 12.5

25.0 and 50 μmol/L remarkably inhibited the proliferation of A549 human lung andenocarcinoma cells (

0.05). Combined resveratrol (6.25 to 50 μmol/L) and IGF-Ⅰ (10 nmol/L) could inhibit the IGF-ⅠR-induced proliferation of A549 human lung andenocarcinoma cells

especially at the concentrations of 25.0 and 50.0 μmol/L (

0.05). Resveratrol could effectively down-regulate the expression of IGF-ⅠR mRNA and protein. Conclusion Resveratrol can effectively inhibit the IGF-ⅠR-induced proliferation of A549 human lung adenocarcinoma cells by down-regulating the expression of IGF-ⅠR mRNA and protein.

关键词

白藜芦醇胰岛素样生长因子-1胰岛素样生长因子-1受体人肺腺癌细胞A549

Keywords

resveratrolIGF-ⅠIGF-ⅠRA549 human lung adenocarcinoma cells

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