Dopamine D<sub>1</sub>-like receptors regulate renalase in renal proximal tubule cells through PKC pathway <em>in vitro</em>

Wang Shaoxiong; Yang Jian; Chen Caiyu; Han Yu; Ren Hongmei; He Duofen; Zeng Chunyu

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Dopamine D₁-like receptors regulate renalase in renal proximal tubule cells through PKC pathway in vitro

更新时间：2025-01-15

- Dopamine D₁-like receptors regulate renalase in renal proximal tubule cells through PKC pathway in vitro
- Journal of Army Medical University Vol. 33, Issue 24, Pages: 2558-2561(2011)
- 作者机构：
  
  第三军医大学大坪医院野战外科研究所：心血管内科，重庆市心血管病研究所，营养科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.61;R329.26;R394.2
- Published：2011，
  
  Published Online：30 December 2011，
- 稿件说明：
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WANG SHAOXIONG, YANG JIAN, CHEN CAIYU, et al. Dopamine D₁-like receptors regulate renalase in renal proximal tubule cells through PKC pathway in vitro. [J]. Journal of army medical university, 2011, 33(24): 2558-2561.
DOI：

WANG SHAOXIONG, YANG JIAN, CHEN CAIYU, et al. Dopamine D₁-like receptors regulate renalase in renal proximal tubule cells through PKC pathway in vitro. [J]. Journal of army medical university, 2011, 33(24): 2558-2561. DOI：

摘要

目的研究多巴胺D

类受体对Wistar-Kyoto（WKY）大鼠肾近曲小管上皮细胞（renal proximal tubule

RPT）中肾胺酶（renalase）的表达与活性的影响及其作用机制。方法以WKY大鼠RPT细胞株为实验对象，采用RT-PCR方法检测D

类受体激动剂fenoldopam对renalase mRNA转录水平的影响，免疫印迹法（Western blot）测定蛋白表达的变化，尼克酰胺腺嘌呤二核苷酸（nicotinamide adenine dinucleotide，NADH）氧化法检测renalase活性。结果多巴胺D

类受体激动剂fenoldopam （10

-6

mol/L）作用24 h可显著上调WKY大鼠RPT细胞renalase 的mRNA[（1.09±0.07）

（0.67±0.11）

]

和蛋白[（1.12±0.10）

（0.73±0.05）

]

表达水平（

0.05）。与对照组相比，fenoldopam（10

-6

mol/L）刺激24 h后，RPT细胞renalase的NADH氧化活性也明显增高[（3.98±0.06）

（3.55±0.13）

0.05

]

。fenoldopam上调WKY大鼠RPT细胞的renalase的转录、蛋白合成和活性，并呈一定的浓度依赖性。fenoldopam对renalase的上调作用可以被蛋白激酶C抑制剂( protein kinase C inhibitor

PKCI，即PKC抑制肽19-31， 10

-6

mol/L）所阻断。结论多巴胺D

类受体能够增强WKY大鼠RPT细胞中renalase的表达水平与活性，该调解作用与PKC信号途径有关。

Abstract

Objective To determine the effect of dopamine D

-like receptors on the expression and activity of renalase in

renal proximal tubule（RPT）cells. Methods Effect of fenoldopam

an agonist of dopamine D

-like receptors on renalase expression in RPT cells from Wistar-Kyoto（WKY） rats was detected by Western blotting for protein expression

RT-PCR for mRNA level and Nicotinamide adenine dinucleotide (NADH) oxidation method for renalase activity. Results In WKY RPT cells

fenoldopam treatment at 10

-6

mol/L for 24 h significantly increased the renalase expression at mRNA and protein levels（1.09±0.07

0.67±0.11

1.12±0.10

0.73±0.05

0.05）. It also enhanced the activity of renalase in RPT cells after treatemnt （3.98±0.06

3.55±0.13

0.05). Fenoldopam upregulated renalase at transcription and protein synthesis levels

and improved its activity in a dose dependent manner. This effect was blocked by the protein kinase C inhibitor (PKCI

PKC inhibitor peptides 19-31）at the concentration of 10

-6

mol/L（

0.05）. Conclusion D

-like receptors improve renalase expression and activity in RPT cells from WKY rats

which may be related to PKC pathway.

关键词

多巴胺D1类受体肾胺酶肾近曲小管上皮细胞蛋白激酶C

Keywords

D1-like receptorsrenalaserenal proximal tubule cellsprotein kinase C

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Dopamine D1-like receptors regulate renalase in renal proximal tubule cells through PKC pathway in vitro

DOI：

摘要

Abstract

关键词

Keywords

references

Dopamine D₁-like receptors regulate renalase in renal proximal tubule cells through PKC pathway in vitro