Effect of liver X receptor agonist TO901317 on astrocyte in hippocampal dentate gyrus in adult mice

Huang Wei; Guo Liang; Xu Pei; Fan Xiaotang; Xu Haiwei

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Effect of liver X receptor agonist TO901317 on astrocyte in hippocampal dentate gyrus in adult mice

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- Effect of liver X receptor agonist TO901317 on astrocyte in hippocampal dentate gyrus in adult mice
- Journal of Army Medical University Vol. 33, Issue 24, Pages: 2550-2553(2011)
- 作者机构：
  
  第三军医大学：基础医学部生理学教研室，组织与胚胎学教研室，重庆市神经科学研究所，西南医院全军眼科中心
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.28;R338.26;R34
- Published：2011，
  
  Published Online：30 December 2011，
- 稿件说明：
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HUANG WEI, GUO LIANG, XU PEI, et al. Effect of liver X receptor agonist TO901317 on astrocyte in hippocampal dentate gyrus in adult mice. [J]. Journal of army medical university, 2011, 33(24): 2550-2553.
DOI：

HUANG WEI, GUO LIANG, XU PEI, et al. Effect of liver X receptor agonist TO901317 on astrocyte in hippocampal dentate gyrus in adult mice. [J]. Journal of army medical university, 2011, 33(24): 2550-2553. DOI：

摘要

目的观察肝脏X受体(liver X receptor，LXR) 激动剂TO901317对成年小鼠海马齿回（dentate gyrus， DG）星形胶质细胞数量以及海马齿回颗粒下区(dentate gyrus subgranular zone

DG-SGZ)星形胶质细胞放射状突起的作用。方法 3个月龄健康雄性C57B/L6小鼠采用TO901317激动剂灌胃1周，采用免疫组化检测小鼠海马星形胶质细胞的数量和形态学变化，采用RT-PCR检测海马LXRα、LXRβ、Klf-9、ABCA1、ABCG1和apoE的表达。测量肝脏大小和质量。结果 TO901317激动剂灌胃1周后，海马齿回胶质纤维酸性蛋白(glial fibrillary acidic protein

GFAP)阳性的星形胶质细胞数量显著减少（

0.05），而海马DG-SGZ区GFAP阳性的放射状纤维显著增多（

0.01）；小鼠海马LXRα表达无显著变化（

>0.05），而LXRβ表达显著下降（

0.05）

Klf-9、ABCA1、ABCG1和apoE表达显著升高（

0.05）。灌注TO901317后小鼠肝脏显著肿大，质量较DMSO对照组显著增加（

0.01）。结论 TO901317通过LXRβ受体调节Klf-9、ABCA1、ABCG1、apoE的表达，从而显著增加海马神经发生区GFAP长纤维的延伸。

Abstract

Objective To determine the effect of TO901317

the agonist of liver X receptor

on the number of astrocytes in hippocampal dentate gyrus (DG) and on radial processes in the dentate gyrus subgranular zone (DG-SGZ) in adult mice. Methods TO901317 (40 mg/kg) was intragastrically administrated to the healthy female C57B/L6 mice for 7

d. The number of GFAP positive cells and radial-like fibers in the hippocampus was detected by immunohistochemistry. The expression of LXRα

LXRβ

Klf-9

ABCA1

ABCG1 and apoE was detected by RT-PCR. The size and weight of the liver were also observed. Results After 1 week’s TO901317 treatment

the number of GFAP positive cells in the DG was decreased markedly (

0.05)

while the GFAP positive radial-like fiber in the SGZ was increased significantly (

0.01). No change was found in the expression of LXRα

while that of LXRβ was decreased (

0.05). The expression of Klf-9

ABCA1

ABCG1 and apoE was increased significantly after TO901317 administration (

0.05). Significant increases of the liver size and weight were observed in TO901317 group when compared with those in DMSO group (

0.01). Conclusion Through LXRβ receptor

TO901317 modulates the expression of Klf-9

ABCA1

ABCG1 and apoE

and improves the outgrowth of radial-like process of astrocytes in the DG-AGZ.

关键词

肝脏X受体星形胶质细胞海马肝脏神经发生

Keywords

liver X receptorastrocytehippocampusliverneurogenesis

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