Costimulatory molecule VSIG4 exclusively expressed on macrophages inhibits proliferation and activation in T lymphocytes

Li Ming; Wang Yiqin; Li Yan; Tao Guangli; Pang Qi; Yuan Fahuan

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Costimulatory molecule VSIG4 exclusively expressed on macrophages inhibits proliferation and activation in T lymphocytes

更新时间：2025-01-15

- Costimulatory molecule VSIG4 exclusively expressed on macrophages inhibits proliferation and activation in T lymphocytes
- Journal of Army Medical University Vol. 33, Issue 22, Pages: 2335-2339(2011)
- 作者机构：
  
  第三军医大学新桥医院肾内科，全军肾脏病中心，重庆市肾病研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392-33;R392.12
- Published：2011，
  
  Published Online：30 November 2011，
- 稿件说明：
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LI MING, WANG YIQIN, LI YAN, et al. Costimulatory molecule VSIG4 exclusively expressed on macrophages inhibits proliferation and activation in T lymphocytes. [J]. Journal of army medical university, 2011, 33(22): 2335-2339.
DOI：

LI MING, WANG YIQIN, LI YAN, et al. Costimulatory molecule VSIG4 exclusively expressed on macrophages inhibits proliferation and activation in T lymphocytes. [J]. Journal of army medical university, 2011, 33(22): 2335-2339. DOI：

摘要

目的探讨巨噬细胞表达共刺激因子VSIG4（V-set and immunoglobulin domain-containing protein 4）对T细胞增殖与活化的影响。方法分别从VSIG4基因敲除型(VSIG4

-/-

)小鼠和同窝的野生型（VSIG4

+/+

）小鼠（遗传背景为C57BL/6）腹腔收集巨噬细胞，在CD3单抗的刺激下与T细胞共培养，设为VSIG4

-/-

/T组及VSIG4

+/+

/T组，经 CD3单抗刺激的T细胞单独培养设为对照组，未经 CD3单抗刺激的 T 细胞单独培养设为空白组。

H-TdR掺入法检测细胞增殖;FACS测T细胞的活化标志CD69；酶联免疫吸附实验（ELISA）法检测共培养上清中IL-2、IFN-γ蛋白表达变化，RT-PCR法检测IL-2、IFN-γ基因水平表达变化。结果两共培养组中T细胞

H掺入量均低于对照组（

0.01）；CD69阳性率、IL-2、IFN-γ的蛋白及基因表达同样均低于对照组（

0.05）；且VSIG4

+/+

/T组中T细胞以上指标相对于VSIG4

-/-

/T组下降更加明显，且两组间比较差异显著（

0.05）。结论巨噬细胞特异表达的共刺激因子VSIG4在巨噬细胞与T细胞共培养抑制T细胞增殖活化的过程中发挥了作用。

Abstract

Objective To determine the effect of costimulatory molecules V-set and immunoglobulin domain-containing protein 4 (VSIG4) which is exclusively expressed in the macrophages on the proliferation and activation of T lymphocytes. Methods Mouse peritoneal macrophages from 8-week-old VSIG4 knockout (VSIG4

-/-

) mice and healthy wide-type (VSIG4

+/+

) mice (C57BL/6 mice) were collected

and then co-cultured with T cells respectively under the stimulation of CD3 monoclonal antibody. T cells cultured without macrophages but in the stimulation of CD3 monoclonal antibody served as control

and those without macrophages and CD3 monoclonal antibody as the blank control. Purified T cells was evaluated by [

]

-TdR incorporation assay. Activity of CD69 was tested by FACS. Levels of IL-2 and IFN-γ protein in the supernatants of the co-cultured cells were measured by enzyme-linked immunosorbent assay (ELISA)

and mRNA expression of IL-2 and IFN-γ were detected by RT-PCR. Results In the two co-culture groups

the [

]

-thymidine incorporation

activity of CD69

cell mRNA and protein levels of IL-2 and IFN-γ were significantly lower than those in the control groups (

0.05，

0.01). And these indicators in the VSIG4

+/+

/T group were significantly declined when compared with those of VSIG4

-/-

/T group (

0.05). Conclusion Costimulatory molecule VSIG4 specifically expressed in the macrophage might play a role in the process of inhibiting the proliferation and activation of T lymphocytes co-cultured macrophage.

关键词

巨噬细胞T细胞共刺激分子VSIG4基因敲除小鼠

Keywords

macrophageT cellscostimulatory moleculeV-set and immunoglobulin domain-containing protein 4gene knockout mice

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