Hypoxia induces DNA methylation imbalance in human small cell lung cancer cells H446

Guo Ruiling; Wu Guoming; Ji Fuyun; Li Haidong; Li Wenbi; Pan Feng

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Hypoxia induces DNA methylation imbalance in human small cell lung cancer cells H446

更新时间：2025-01-15

- Hypoxia induces DNA methylation imbalance in human small cell lung cancer cells H446
- Journal of Army Medical University Vol. 33, Issue 20, Pages: 2145-2147(2011)
- 作者机构：
  
  1. 解放军第324医院呼吸内科
  2. 第三军医大学新桥医院全军呼吸内科研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R734.2;R364.4;R730.23
- Published：2011，
  
  Published Online：30 October 2011，
- 稿件说明：
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GUO RUILING, WU GUOMING, JI FUYUN, et al. Hypoxia induces DNA methylation imbalance in human small cell lung cancer cells H446. [J]. Journal of army medical university, 2011, 33(20): 2145-2147.
DOI：

GUO RUILING, WU GUOMING, JI FUYUN, et al. Hypoxia induces DNA methylation imbalance in human small cell lung cancer cells H446. [J]. Journal of army medical university, 2011, 33(20): 2145-2147. DOI：

摘要

目的研究缺氧对人小细胞肺癌H446细胞基因组DNA甲基化水平的影响。方法体外培养人小细胞肺癌细胞H446，置于缺氧（3%O

）条件下培养，分别于0、12、24、48、72 h后提取细胞基因组DNA，用甲基化敏感性随机引物PCR（MS-AP-PCR）方法检测基因组DNA甲基化状态。结果在缺氧状态下，H446细胞经酶解后的扩增谱带明显增多，且荧光强度较强，以24 h后明显，表明H446细胞基因组DNA甲基化水平在缺氧24 h后即发生明显升高，主要表现为多位点的异常甲基化。结论缺氧可能是造成肿瘤细胞内甲基化失衡的重要原因之一。

Abstract

Objective To investigate the influence of hypoxia on the methylation level of genomic DNA in human small cell lung cancer H446 cells. Methods H446 cells were cultured under the hypoxic condition (3%O

) for different time periods (0

48 and 72 h respectively). Those cultured under normal condition served as control. The genomic DNA methylation level was detected by methylation sensitivity random primers PCR (MA-AS-PCR) after DNA extraction. Results Compared with the normoxic control group

the quantity and fluorescence intensity of amplified products of hypoxic experimental group were much higher

especially after 24 h. This indicated that the genomic DNA methylation level in the H446 cells was signi

ficantly changed when were treated by hypoxia

and represented mainly as multifocal abnormal methylation. Conclusion Hypoxia might be one of the key causer inducing unbalanced methylation in cancer cells.

关键词

缺氧癌，小细胞DNA甲基化细胞系肿瘤

Keywords

hypoxiacarcinoma small cellDNA methylationcell line tumor

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