Preparation of bromfenac sodium/chitosan nanoparticles loaded fibrin-binding amniotic membrane and its effect on rabbit corneal neovascularization <em>in vitro</em>

Wu Guiyu; Zhao Min; Ji Yan

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Preparation of bromfenac sodium/chitosan nanoparticles loaded fibrin-binding amniotic membrane and its effect on rabbit corneal neovascularization in vitro

更新时间：2025-01-15

- Preparation of bromfenac sodium/chitosan nanoparticles loaded fibrin-binding amniotic membrane and its effect on rabbit corneal neovascularization in vitro
- Journal of Army Medical University Vol. 35, Issue 6, Pages: 495-499(2013)
- 作者机构：
  
  重庆医科大学附属第一医院眼科，重庆市眼科学重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R772.2;R944.9;R318.08
- Published：2013，
  
  Published Online：30 March 2013，
- 稿件说明：
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WU GUIYU, ZHAO MIN, JI YAN. Preparation of bromfenac sodium/chitosan nanoparticles loaded fibrin-binding amniotic membrane and its effect on rabbit corneal neovascularization in vitro. [J]. Journal of army medical university, 2013, 35(6): 495-499.
DOI：

WU GUIYU, ZHAO MIN, JI YAN. Preparation of bromfenac sodium/chitosan nanoparticles loaded fibrin-binding amniotic membrane and its effect on rabbit corneal neovascularization in vitro. [J]. Journal of army medical university, 2013, 35(6): 495-499. DOI：

摘要

目的制备一种具有双重缓释功能的新型生物复合材料溴芬酸钠/壳聚糖纳米粒胶联羊膜(BF/CS-FBAM)，初步研究其体外缓释性能及对角膜新生血管的影响。方法采用离子胶联法制备溴芬酸钠/壳聚糖纳米粒并观察其表征，将载药纳米粒混入纤维蛋白胶中，再将混合物喷涂到羊膜表面制成BF/CS-FBAM，并进行形态学和体外释药观察，最后通过兔角膜碱烧伤模型观察BF/CS-FBAM对角膜新生血管的影响，用实时荧光定量PCR方法检测术后角膜中VEGF的表达。结果溴芬酸钠/壳聚糖纳米粒呈球形，表面光滑，大小均一，其粒径为(548.7±14.4)nm，Zeta电位为(29.8±2.2) mV，包封率为(67.11±4.56)%，载药量为(57.71±5.38)%。载有纳米药的纤维蛋白胶与羊膜黏合紧密，表面呈网状结构，纳米药充斥其中。在体外，BF/CS-FBAM的释药可达14 d。在碱烧伤模型中，术后BF/CS-FBAM组和BF/CS组在减少炎症反应、抑制新生血管及降低VEGF表达方面都显著优于对照组，且各时间点的差异有统计学意义(

0.05)；同时，术后14 d和21 d BF/CS-FBAM组中VEGF的表达明显低于BF/CS组(

0.05)。结论制备的BF/CS-FBAM作为一种新型生物移植材料可在局部缓慢释放溴芬酸钠，并对角膜新生血管的产生有较好的抑制性。

Abstract

Objective To prepare a novel biological composite material

bromfenac sodium/chitosan nanoparticle loaded fibrin-binding amniotic membrane (BF/CS-FBAM)

with double sustained-released function

and investigate the drug release

in vitro

and its effect on rabbit corneal neovascularization (CNV). Methods The ionotro

pic gelation was used to produce bromfenac sodium/chitosan nanoparticles (BF/CS). After its physic properties were identified

the BF/CS nanoparticles were mixed with fibrin glue (FG)

and then sprayed to the surface of amniotic membrane (AM). After BF/CS-FBAM was obtained

the morphological changes and drug release

in vitro

were observed and measured. The effect of BF/CS-FBAM for CNV was observed in rabbit corneal alkali burn model

meanwhile the expression of vascular endothelial growth factor (VEGF) in the cornea were quantified by reverse transcription-quantitative PCR (RT-qPCR). Results The nanoparticles were smooth and spherical

in a diameter of 548.7±14.4 nm

and had a positive zeta potential of 29.8±2.2 mV. The encapsulation efficiency and loading capacity of BF/CS nanoparticles were (67.11±4.56)% and (57.71±5.38)% respectively. The nanoparticle-loaded fibrin gel was firmly adhered to the AM. Moreover

nanoparticles were coated within FBAM symmetrically

and its surface had a netted structure. The BF released from the BF/CS-FBAM was sustained for approximately 14 d

in vitro

. In rabbit corneal alkali burn model

BF/CS-FBAM group and BF/CS group had better curative effect in attenuating inflammatory reaction

anti-CNV and down-regulating VEGF than control group in every time point after injury (

0.05). Meanwhile

the expression of VEGF in BF/CS-FBAM group was significantly lower than BF/CS group in the 14th and 21st day after injury (

0.05). Conclusion As a novel biological implantable material

the fabricated BF/CS-FBAM locally releases BF in a sustained way and inhibits the generation of CNV.

关键词

溴芬酸钠纤维蛋白胶壳聚糖纳米粒羊膜角膜新生血管

Keywords

bromfenac sodiumfibrin gluechitosannanoparticleamniotic membranecorneal neovascularization

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Related Institution

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University)

Department of Emergency, 3Center for Joint Surgery, First Affiliated Hospital, Army Medical University (Third Military Medical University)

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Department of Ophthalmology, Guizhou Provincial People’s Hospital

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Preparation of bromfenac sodium/chitosan nanoparticles loaded fibrin-binding amniotic membrane and its effect on rabbit corneal neovascularization in vitro

DOI：

摘要

Abstract

关键词

Keywords

references