Highly sensitive detection of tumor markers CEA and CYFRA21-1 for lung cancer based on gold nanoparticle probes and protein chip

Wang Wentao; Jia Chunping; Jin Qinghui; Zhao Jianlong; Zhang Guojun

您当前的位置：

首页 >

文章列表页 >

Highly sensitive detection of tumor markers CEA and CYFRA21-1 for lung cancer based on gold nanoparticle probes and protein chip

更新时间：2025-01-15

- Highly sensitive detection of tumor markers CEA and CYFRA21-1 for lung cancer based on gold nanoparticle probes and protein chip
- Journal of Army Medical University Vol. 35, Issue 6, Pages: 500-503(2013)
- 作者机构：
  
  1. 郑州大学第一附属医院呼吸内科
  2. 中国科学院上海微系统与信息技术研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R318;R730.4;R734.2
- Published：2013，
  
  Published Online：30 March 2013，
- 稿件说明：
移动端阅览
WANG WENTAO, JIA CHUNPING, JIN QINGHUI, et al. Highly sensitive detection of tumor markers CEA and CYFRA21-1 for lung cancer based on gold nanoparticle probes and protein chip. [J]. Journal of army medical university, 2013, 35(6): 500-503.
DOI：

WANG WENTAO, JIA CHUNPING, JIN QINGHUI, et al. Highly sensitive detection of tumor markers CEA and CYFRA21-1 for lung cancer based on gold nanoparticle probes and protein chip. [J]. Journal of army medical university, 2013, 35(6): 500-503. DOI：

摘要

目的构建基于纳米金探针和蛋白芯片的快速、多肿瘤标志物高灵敏检测体系。方法将待检测的肿瘤标志物捕获抗体（捕获待测抗原）结合在醛基化修饰的玻片上；用检测抗体制备纳米金探针；经过免疫反应形成三明治夹心结构（蛋白芯片-目标蛋白-纳米金探针）。利用纳米金沉积的方法进行染色，通过肉眼或显微镜观察显色结果。结果该蛋白检测体系在1 h内可检测两种肿瘤标志物，其中CEA可检测到最低浓度为45 pg/mL，CYFRA21-1可检测到90 pg/mL，与传统的酶联免疫吸附法(ELISA)相比，检测灵敏度大大提高。并利用此体系，检测肺癌患者及正常人血清，结果与临床所用电化学分析方法一致。结论该检测体系操作简单、方便、快速，同时具有高灵敏度、多指标联合检测等优点，在临床蛋白检测中具有重要的价值和应用前景。

Abstract

Objective To construct a rapid and highly sensitive detection system based on gold nanoparticle probes and protein chip. Methods The capture antibody (capture for target antigen) was combined to the aldehyde modified glass

and the nanoparticle probes were prepared using detection antibody. Then

the sandwich structures (protein chip-the target protein-gold nanoparticle probes) were created through the interaction between the antibody and antigen

and dyed by the gold deposition solution. The results were observed with the naked eye or by microscopy. Results For this protein detection system

CEA and CYFRA21-1 could be detected as low as 45 pg/mL and 90 pg/mL respectively in 1 h. The sensitivity was improved greatly

compared with the traditional ELISA method. Using this system

the lung cancer patients and normal people detection results were consistent with clinical used method (electrochemical analysis). Conclusion This system for CEA and CYFRA21-1 detection is an easy

rapid

convenient method with high sensitivity and multiple detection

which might have an important value and great application prospect in the clinical protein detection.

关键词

纳米金探针蛋白芯片纳米金沉积肿瘤标志物肺癌

Keywords

gold nanoparticle probeprotein chipgold depositiontumor markerslung cancer

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Combined determination of tumor markers in serum and bronchoalveolar lavage fluid for lung cancer diagnosis

Effect of 3-bromopyruvate combined with cisplatin on inhibiting growth of lung cancer A549 cells

Related Author

DENG Li-ping

DONG Wen

DU Yan-ping

JIANG Xing-tang

ZHAO Binyu

ZHANG Junlei

WANG Jiaqi

RUAN Yan

Related Institution

Department of Respiratory Diseases, Affiliated Zhongshan Hospital of Xiamen University

Department of Ophthalmology, First Affiliated Hospital, Army Medical University (Third Military Medical University)

Department of Physiology, 2Department of Histology and Embryology, 3Teaching Experiment Center, College of Basic Medical Sciences, Army Medical University (Third Military Medical University)

Key Laboratory of Optoelectronic Technology and Systems of Ministry of Education, Defense Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University

Center of Clinical Laboratory, Second Affiliated Hospital, Army Medical University (Third Military Medical University)

⁰