Sodium valproate inhibits proliferation in rat neural stem cells through p21 pathway

您当前的位置：

首页 >

文章列表页 >

Sodium valproate inhibits proliferation in rat neural stem cells through p21 pathway

更新时间：2025-01-15

|

- Sodium valproate inhibits proliferation in rat neural stem cells through p21 pathway
- Journal of Army Medical University Vol. 35, Issue 6, Pages: 487-490(2013)
- 作者机构：
  
  第三军医大学西南医院神经外科,全军神经外科研究所，全军神经创伤防治重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.28;R971.6;R322.8
- Published：2013，
  
  Published Online：30 March 2013，
- 稿件说明：
移动端阅览
HUANG LEI, CHU WEIHUA, YUAN JICHAO, et al. Sodium valproate inhibits proliferation in rat neural stem cells through p21 pathway. [J]. Journal of army medical university, 2013, 35(6): 487-490.
DOI：

HUANG LEI, CHU WEIHUA, YUAN JICHAO, et al. Sodium valproate inhibits proliferation in rat neural stem cells through p21 pathway. [J]. Journal of army medical university, 2013, 35(6): 487-490. DOI：

摘要

目的探讨丙戊酸钠(sodium valproate，VPA)对体外培养的成年雌大鼠脊髓神经干细胞(neural stem cells，NSCs)增殖的影响。方法采用不同浓度的VPA(10

-5

、10

-4

、10

-3

、10

-2

、10

-1

、1、10 mmol/L)作用于NSCs，CCK-8法检测在不同时间点(0、24、48、72 h)对细胞增殖的影响；VPA(10

-5

、1 mmol/L)作用于NSCs 48 h后，流式细胞仪测定细胞周期分布，PCR测定p21在基因水平的表达，Western blot测定p21在蛋白质水平的表达。结果 CCK-8检测显示，当VPA浓度＞10

-5

mmol/L时，体外培养的成年大鼠脊髓NSCs的增殖受到明显抑制，且具有时间依赖性。流式细胞仪细胞周期检测显示，同样浓度下，VPA可阻滞NSCs由G

0

/G

1

期向S期转换，表现为G

0

/G

1

期细胞增多，S期细胞减少，G

2

/M期细胞减少。PCR检测发现VPA可促进p21基因水平的表达。Western blot检测发现VPA可促进p21蛋白质水平的表达，各组间差异均有统计学意义(

P

<

0.05)。结论 VPA可能通过促进p21表达，使NSCs阻滞于G

0

/G

1

期，最终抑制NSCs增殖。

Abstract

Objective To determine the effect of sodium valproate (VPA) on the proliferation and cell cycle in adult female rat spinal neural stem cells (NSCs)． Methods Cell proliferation was assessed by CCK-8 assay after the cells were treated with VPA at different concentrations of 10

-5

10

-4

10

-3

10

-2

10

-1

1 or 10 mmol/L for 0

24

48 or 72 h. After NSCs were treated with VPA at a dose of 10

-5

and 1 mmol/L for 48 h

cell cycle was analyzed by flow cytometry and the expression of p21(cyclin-dependent kinase inhibitor) was detected by PCR and Western blot analysis. Results CCK-8 staining colorimetry showed that the proliferation of NSCs was markedly inhibited in a time-dependent manner when the concentration of VPA was more than 10

-5

mmol/L. Flow cytometry indicated more cultured NSCs were arrested in the G

0

/G

1

phase and fewer at the S phase after being treated with VPA

which indicated that VPA arrested the transition of NSCs from G

0

/G

1

phase to S phase. PCR and Western blot analysis indicated that VPA enhanced the expression of p21 at mRNA and protein levels(

P

<

0.05)． Conclusion VPA may arrest NSCs at G

0

/G

1

by increasing the expression of p21

and then finally inhibit the proliferation of NSCs．

关键词

丙戊酸钠脊髓神经干细胞增殖p21

Keywords

sodium valproatespinal cordneural stem cellsproliferationp21

references

0

Views

0

下载量

0

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Related Articles

Roles of β-catenin gene in differentiation of rat spinal cord derived neural stem cells

Effects of ecdysterone on proliferation and differentiation of rat neural stem cells in vitro

Related Author

Li Cheng

Bi Yang

Wang Jiuling

Nan Guoxin

CHU Wei-hua

ZHAN Meng-xiong

ZHU Gang

CHEN Zhi

Related Institution

Laboratory of Stem Cells of Chongqing Pediatrics Institute, Key Laboratory of Child Development and Disorders of Ministry of Education, Chongqing Key Laboratory of Pediatrics, Chongqing International Science and Technology Cooperation Base for Child Development and Disorders, the Children’s Hospital of Chongqing Medical University

Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing

Institute of Life Science, Northeast Normal University

The First Clinical Medical College, Lanzhou University

Department of Anesthesiology, the First Hospital of Lanzhou University

⁰