Preparation and <em>in vitro</em> release of albumin nanoparticles loaded with isoniazid and rifampicin

Ma Rong; Chen Zhen; Zhang Huiyong; Yang Xiaoying; Wang Wenping; Ge Zhaohui

您当前的位置：

首页 >

文章列表页 >

Preparation and in vitro release of albumin nanoparticles loaded with isoniazid and rifampicin

更新时间：2025-01-15

- Preparation and in vitro release of albumin nanoparticles loaded with isoniazid and rifampicin
- Journal of Army Medical University Vol. 35, Issue 21, Pages: 2336-2339(2013)
- 作者机构：
  
  宁夏医科大学：研究生院,药学院,总医院药剂科，总医院骨科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R912;R944.9;R978.3
- Published：2013，
  
  Published Online：15 November 2013，
- 稿件说明：
移动端阅览
MA RONG, CHEN ZHEN, ZHANG HUIYONG, et al. Preparation and in vitro release of albumin nanoparticles loaded with isoniazid and rifampicin. [J]. Journal of army medical university, 2013, 35(21): 2336-2339.
DOI：

MA RONG, CHEN ZHEN, ZHANG HUIYONG, et al. Preparation and in vitro release of albumin nanoparticles loaded with isoniazid and rifampicin. [J]. Journal of army medical university, 2013, 35(21): 2336-2339. DOI：

摘要

目的研究载异烟肼(isoniazid

INH)、利福平(rifampicin

RFP)牛血清白蛋白(bovine serum albumin

BSA)纳米粒的制备及体外释药特性。方法以白蛋白为载体聚乳酸为骨架，采用改良自乳化-溶剂扩散法制备纳米粒。采用透射电镜观察纳米粒形态；激光粒度衍射分析仪测定纳米粒粒径分布；高效液相色谱法测定纳米粒的载药量和包封率；动态膜透析法观察载药纳米粒的体外释药特性。结果制备的INH-RFP-BSA-NPs电镜下观察外观圆整，大小均匀、分散性好，粒径为（60.5±4.6）nm。异烟肼、利福平的载药量分别为19.8%、20.1%，包封率分别为87.8%、98%。INH至6 d时累计释放97.02%，RFP至5d时完全释放。结论以白蛋白和聚乳酸为载药基质，采用改良自乳化-溶剂扩散法可制备出粒径小、载药量和包封率较高的载异烟肼、利福平白蛋白纳米粒

体外释药显示载异烟肼、利福平白蛋白纳米粒具有良好的缓释作用。

Abstract

Objective To prepare of bovine serum albumin nanoparticles loaded with isoniazid and rifampicin (INH-RFP-BSA-NPs)and investigate its

in vitro

release characteristics. Methods INH-RFP-BSA-NPs were prepared by modified self-emulsion solvent diffusion method. Albumin and polylactic acid was used as a carrier as well as skeleton structure. Transmission electron microscopy was used to observe the morphology of INH-RFP-BSA-NPs. The nanoparticle’s size and distribution of INH-RFP-BSA-NPs were performed by submicron particle size analyzer. High performance liquid ch

romatography (HPLC) was used to measure the drug loadings and the coating rate of INH-RFP-BSA-NPs. Dynamic membrane dialysis was used to study the

in vitro

release characteristics of INH-RFP-BSA-NPs. Results The INH-RFP-BSA-NPs were smooth

sphere-like

relatively uniform in size and good dispersion. The submicron particle size analyzer showed the diameter was 60.5±4.6 nm. The drug loading and entrapment efficiency of isoniazid were 19.8% and 87.8% respectively

and those of rifampicin were 20.1% and 98% respectively

INH cumulative release of 97.02% to 6 d

RFP to 5 d fully released. Conclusion INH-RFP-BSA-NPs can be prepared by drug-loading with albumin and polylactic acid

matrix modified self-emulsion solvent diffusion

with higher encapsulation efficiency

higher drug loading

and smaller particle size.

In vitro

drug release shows INH-RFP-BSA-NPs has good slow release effect.

关键词

白蛋白异烟肼利福平纳米粒体外释药

Keywords

albuminisoniazidrifampicinnanoparticlesin vitro drug release

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Preparation of endostatin-loaded chitosan nanoparticles and antitumor effect of the nanoparticles combined with cisplatin in Lewis lung cancer mice

Preparation and performance of PEGylated chitosan nanoparticles for ocular drug delivery by eye drops

Tumor-specific aggregation of magnetic liposome nanoparticles in tumor-bearing mice after ultrasound-guided intratumor injection of liquid-solid phase-change biomagnetic material

Inhibitory effect of Cubilin antisense RNA on albumin overload in renal tubules in rats

Related Author

DU Yonghong

LI Dairong

FENG Xiaoling

LI Jianhu

LI Gangjing

Wen Qinglian

He Yu

He Wenfeng

Related Institution

State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University

Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University

Department of Oncology, the Affiliated Hospital of Southwest Medical University

Department of Ophthalmology, Central Laboratory, Yongchuan Hospital of Chongqing Medical University

Institute of Ultrasound Imaging, Chongqing Key Laboratory of Ultrasound Molecular Imaging, Chongqing Medical University

⁰

Preparation and in vitro release of albumin nanoparticles loaded with isoniazid and rifampicin

DOI：

摘要

Abstract

关键词

Keywords

references