Expression of RANKL and M-CSF in synovium of CIA rats and intervention of T-614 and methotrexate

Wang Qianqian; Wang Meimei

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Expression of RANKL and M-CSF in synovium of CIA rats and intervention of T-614 and methotrexate

更新时间：2025-01-15

- Expression of RANKL and M-CSF in synovium of CIA rats and intervention of T-614 and methotrexate
- Journal of Army Medical University Vol. 35, Issue 17, Pages: 1813-1817(2013)
- 作者机构：
  
  东南大学：医学院，附属中大医院风湿免疫科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.3;R779.12;R593.22
- Published：2013，
  
  Published Online：15 September 2013，
- 稿件说明：
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WANG QIANQIAN, WANG MEIMEI. Expression of RANKL and M-CSF in synovium of CIA rats and intervention of T-614 and methotrexate. [J]. Journal of army medical university, 2013, 35(17): 1813-1817.
DOI：

WANG QIANQIAN, WANG MEIMEI. Expression of RANKL and M-CSF in synovium of CIA rats and intervention of T-614 and methotrexate. [J]. Journal of army medical university, 2013, 35(17): 1813-1817. DOI：

摘要

目的初步探讨RANKL 和 M-CSF在CIA大鼠关节滑膜的表达及T-614、MTX的干预作用。方法通过鸡Ⅱ型胶原和完全弗氏佐剂混合的胶原乳剂免疫大鼠，建立 CIA 大鼠模型，将 CIA 大鼠随机分为模型对照组、 T-614 组、MTX 组和 T-614+MTX 组。药物干预组大鼠分别用 T-614、MTX 及 T-614 联合 MTX 干预3周。关节炎指数 (arthritis index

AI) 评分评价关节炎程度，HE 染色观察踝关节组织形态学变化，X 线片观察骨侵蚀情况，高频超声观察踝关节并测量滑膜厚度，Western blot 法检测滑膜 RANKL 和 M-CSF 表达。结果与正常对照组相比，模型对照组 AI 评分、病理评分、 X 线评分、超声下滑膜厚度、关节滑膜 RANKL

M-CSF 表达均增加(

0.05)；与模型对照组相比，T-614组、MTX组、T-614 联合MTX组上述观察指标均降低(

0.05)；与T-614组、MTX组相比，T-614 联合MTX组上述观察指标均降低(

0.05)；T-614组和MTX组之间上述观察指标差异无统计学意义（

>0.05）。结论 RANKL 和 M-CSF 参与了CIA大鼠关节滑膜病变的发生、发展，T-614、MTX可能通过减少细胞因子 RANKL

M-CSF 的生成来延缓关节滑膜病变的进展，T-614与MTX具有一定的协同效应。

Abstract

Objective To investigate the expression of RANKL and M-CSF in the synovium of collagen-induced arthritis (CIA) rats and the intervention of T-614 and methotrexate (MTX) on their expression. Methods The CIA animal models (

=40) were established by injecting type Ⅱ collagen and complete Freud’s adjuvant

and then were divided randomly int

o 4 groups

including a model group (

=10)

a MTX group (

=10)

a T-614 group and a MTX+T-614 group (

=10). Arthritis was evaluated by arthritis index (AI). The expression of RANKL and M-CSF in the synovium was detected by Western blotting. The synovial pathological score of ankle joints

the radiographic changes and synovial thickness were evaluated. Results The arthritis index

the expression of RANKL and M-CSF in the synovium

the synovial pathological score

the X-ray score and the synovial thickness were increased in the model control group

the MTX group

the T-614 group and the MTX+T-614 group as compared to the normal control group (

0.05). Compared with the model control group

all above indices were significantly decreased in the MTX group

the T-614 group and the MTX+T-614 group (

0.05). Compared with the MTX group and the T-614 group

all above indices were decreased in the MTX+T-614 group (

0.05). No difference was found in these indices between the MTX and T-614 groups (

>0.05). Conclusion RANKL and M-CSF are involved in the occurrence and development of joint synovitis in CIA rats. T-614 and MTX can delay the inflammation and bone/cartilage destruction in joint synovitis by synergistically suppressing the expression of RANKL and M-CSF.

关键词

胶原诱导性关节炎大鼠艾拉莫德甲氨蝶呤

Keywords

collagen-induced arthritisratsiguratimodmethotrexate

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