Establishment of human hepatocellular carcinoma cell line stably over-expressing Mov10 and its biological behaviors

您当前的位置：

首页 >

文章列表页 >

Establishment of human hepatocellular carcinoma cell line stably over-expressing Mov10 and its biological behaviors

更新时间：2025-01-15

|

- Establishment of human hepatocellular carcinoma cell line stably over-expressing Mov10 and its biological behaviors
- Journal of Army Medical University Vol. 36, Issue 6, Pages: 583-586(2014)
- 作者机构：
  
  1. 重庆医科大学：附属第二医院感染科，病毒性肝炎研究所
  2. 泸州医学院附属医院感染科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R73-351;R730.2;R735.7
- Published：2014，
  
  Published Online：30 March 2014，
- 稿件说明：
移动端阅览
LIU MINHUI, TONG SHIWEN, SHENG YUNJIAN, et al. Establishment of human hepatocellular carcinoma cell line stably over-expressing Mov10 and its biological behaviors. [J]. Journal of army medical university, 2014, 36(6): 583-586.
DOI：

LIU MINHUI, TONG SHIWEN, SHENG YUNJIAN, et al. Establishment of human hepatocellular carcinoma cell line stably over-expressing Mov10 and its biological behaviors. [J]. Journal of army medical university, 2014, 36(6): 583-586. DOI：

摘要

目的构建稳定表达Mov10蛋白的HepG2细胞株，探讨Mov10在肝癌细胞增殖和侵袭等生物学行为中的作用。方法将含Mov10基因的真核表达载体pCMV-Mov10-GFP质粒，采用脂质体介导法转染至人肝癌细胞系HepG2，经过G418抗性筛选阳性克隆。采用qPCR、Western blot技术检测Mov10的表达，免疫荧光技术观察稳定株Mov10的细胞分布。通过MTS、平板克隆形成试验、体外侵袭能力检测观察稳定细胞株的增殖情况、克隆形成能力和侵袭能力。结果成功构建了表达Mov10蛋白的稳定细胞株HepG2-Mov10。内源性及外源性Mov10蛋白主要分布于细胞质。稳定细胞株HepG2-Mov10较对照组细胞增殖速度快（

P

<

0.05），体外侵袭能力强（

P

<

0.05）。稳定细胞株HepG2-Mov10、HepG2-GFP和细胞株HepG2的克隆形成能力分别为（56.7±17.3）%、（38.3±12.1）%、（31.3±8.1）%，HepG2-Mov10细胞与后两者间差异有统计学意义（

P

<

0.05）。结论成功构建稳定表达Mov10的细胞株HepG2-Mov10，发现Mov10能促进肝癌细胞增殖并提高克隆形成能力和侵袭能力。

Abstract

Objective To establish a cell line stably expressing Moloney leukemia virus 10 (Mov10) protein in HepG2 cells and investigate the role of Mov10 in the cell proliferation and invasion. Methods The eukaryotic expression plasmid pCMV-Mov10-GFP was transfected into the HepG2 cells mediated by lipofectamine and then selected with G418. The mRNA and protein expression of Mov10 was detected by qPCR and Western blotting

respectively. Immun

ofluorescence assay was used to analyze the location of Mov10. The proliferation and colony-formation ability was measured by MTS assay and plate colony-formation assay. Cell invasion assay was performed

in vitro

. Results Our established HepG2-Mov10 Cells stably expressed Mov10 protein. Endogenous and exogenous Mov10 protein was mostly distributed in the cytoplasm. HepG2-Mov10 cells had significantly higher proliferative rate and stronger ability of invasion than HepG2 cells (

P

<

0.05). Colony forming efficiency was (56.7±17.3)%

(38.3±12.1)%

(31.3±8.1)% respectively in HepG2-Mov10

HepG2-GFP and HepG2 cells

with the fromer significantly higher than the other 2 cell lines (

P

<

0.05). Conclusion A hepatoma stable cell line over-expressing Mov10 is successfully established. Stable overexpression of Mov10 protein obviously promotes cell proliferation

colony formation and invasion.

关键词

癌，肝细胞Mov10质粒稳定细胞株

Keywords

hepatocellular carcinomaMov10plasmidstable cell line

references

0

Views

0

下载量

0

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Related Articles

Construction and identification of plasmid mediated RNA interference targeting Nogo-66 receptor

Construction and identification of p27Kip1 shRNA expression plasmid and its functional investigation

ShRNA on histone acetylation induces unusual cardiac-specific protein expression in MSCs differentiation into cardiomyocytes

RNAi silenced MRP2 reverses drug resistance of colorectal cancer cell line to vinblastine

Expression and significance of Raf kinase inhibitor protein in hepatocellular carcinoma: report of 72 cases

Related Author

CAI Ming-chun

FAN You-ming

张光红

ZHANG Guang-hong

ZHANG Jin-xiang FAN Ke-shun

WANG Yong

ZHANG Ming-chang

HUANG Yu-kan

Related Institution

Joint Shantou International Eye Center of Shantou University and Chinese University of Hong Kong

Department of Pathophysiology, College of High Altitude Military Medicine, Third Military Medical University

Department of Ophthalmology, Department of Emergency, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology

Wuhan Ai’er Eye Hospital

Pediatric Research Institute, Department of Cardiology, Children’s Hospital, Chongqing Medical University

⁰