Objective        To prepare a thermosensitive

in situ

gel system containing honokiol inclusion complex (HK-HPCD-Gel) for ophthalmic drug delivery and identify its characteristics.       Methods        The honokiol inclusion complex (HK-HPCD) were prepared by solution mixing method. Poloxamer 407 (P407) and Poloxamer 188 (P188) were used as gel matrix

and the

in situ

gel system of HK-HPCD-Gel was obtained by cold-dissolving method. And

in vitro

release and erosion behavior studies were carried out to evaluate the characteristics of the system

.       Results        The formulation was freely flowing liquid at room temperature with a viscosity of 0.189 4±0.099 9 Pa·s and converted to a firm gel at 34.2 ℃ with a higher viscosity of 2.082 2±0.617 2 Pa·s. The

in vitro

release and erosion study indicated that the membraneless method was more appropriate for

in vitro

studies of gels

and the HPCD enhanced the dissolution rate of HK.       Conclusion        The

in situ

gel system is able to improve the dissolution of drugs and prolongs the residence time of the formulation

which shows great potential in ocular application.