Cancer-associated fibroblasts promote the growth of non-small cell lung cancer by secreting interleukin-6

Liu Ming; Xie Mian; Chen Ruchong

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Cancer-associated fibroblasts promote the growth of non-small cell lung cancer by secreting interleukin-6

更新时间：2025-01-15

- Cancer-associated fibroblasts promote the growth of non-small cell lung cancer by secreting interleukin-6
- Journal of Army Medical University Vol. 35, Issue 17, Pages: 1788-1791(2013)
- 作者机构：
  
  广州医科大学附属第一医院广州呼吸疾病研究所，呼吸疾病国家重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.4;R734.2;R730.23
- Published：2013，
  
  Published Online：15 September 2013，
- 稿件说明：
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LIU MING, XIE MIAN, CHEN RUCHONG. Cancer-associated fibroblasts promote the growth of non-small cell lung cancer by secreting interleukin-6. [J]. Journal of army medical university, 2013, 35(17): 1788-1791.
DOI：

LIU MING, XIE MIAN, CHEN RUCHONG. Cancer-associated fibroblasts promote the growth of non-small cell lung cancer by secreting interleukin-6. [J]. Journal of army medical university, 2013, 35(17): 1788-1791. DOI：

摘要

目的探究肿瘤相关成纤维细胞（cancer-associated fibroblasts

CAF）对非小细胞肺癌（non-small cell lung cancer，NSCLC）生长的影响及可能机制。方法分离NSCLC患者CAF及对照正常成纤维细胞(normal fibroblasts

NF)，体外观察CAF和NF对肿瘤细胞生长的影响，检测其分泌的细胞因子白介素-6 （interleukin-6，IL-6）的变化，MTT法检测IL-6对肿瘤细胞生长的影响；裸鼠接种人肺腺癌PC9细胞，同时接种CAF或NF，观察成纤维细胞对肿瘤生长具有影响。结果 CAF能够在体外明显促进PC9细胞的增殖，CAF上清中IL-6表达明显增高，IL-6可促进肿瘤细胞的增殖；移植瘤模型进一步证实CAF对肿瘤生长的促进作用。结论 CAF可通过高分泌IL-6促进NSCLC的增殖。

Abstract

Objective To investigate the role of cancer-associated fibroblasts (CAF) in non-small cell lung cancer (NSCLC) and possible mechanisms. Methods Primary cultures of CAF and normal fibroblasts (NF) from NSCLC patients were established. The effect of CAF on the growth of NSCLC was observed. The expression of interleukin (IL)-6 and related molecules were detected by ELISA. The effect of IL-6 on the proliferation of NSCLC cells was evaluated by MTT assay. Results CAF had greater ability than NF to enhance the tumorigenicity of lung cancer PC9 cell lines. IL-6 was highly expressed in the CAF conditioned media

and further studies identified that IL-6 could promote the growth of NSCLC. CAF also can promote xenograft tumor growth in a mouse model. Conclusion CAF can promote the growth of NSCLC by secretion of IL-6.

关键词

非小细胞肺癌肿瘤相关成纤维细胞白介素-6

Keywords

non-small cell lung cancercancer-associated fibroblastsinterleukin-6

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