Expression of opioid receptors in colon of cathartic colon rats

Wu Jinsong; Liu Baohua; Tong Weidong; Li Fan; Zhang Anping; Fu Tao; Li Chunxue

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Expression of opioid receptors in colon of cathartic colon rats

更新时间：2025-01-15

- Expression of opioid receptors in colon of cathartic colon rats
- Journal of Army Medical University Vol. 35, Issue 21, Pages: 2278-2281(2013)
- 作者机构：
  
  1. 第三军医大学大坪医院野战外科研究所普通外科,全军便秘诊治中心
  2. 白求恩国际和平医院普通外科二科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394.3;R-332;R574.62
- Published：2013，
  
  Published Online：15 November 2013，
- 稿件说明：
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WU JINSONG, LIU BAOHUA, TONG WEIDONG, et al. Expression of opioid receptors in colon of cathartic colon rats. [J]. Journal of army medical university, 2013, 35(21): 2278-2281.
DOI：

WU JINSONG, LIU BAOHUA, TONG WEIDONG, et al. Expression of opioid receptors in colon of cathartic colon rats. [J]. Journal of army medical university, 2013, 35(21): 2278-2281. DOI：

摘要

目的探讨3种阿片受体亚型（MOR、DOR、KOR）在泻剂结肠大鼠模型结肠内的表达变化及意义。方法使用7～8周龄Wistar大鼠20只，体质量（200±20）g，雌雄各半，按随机数字表法分为对照组（

=10）及泻剂结肠组（

=10），单只分笼饲养（环境温度18～28℃，相对湿度40%～80%）。对照组饲以普通饲料，泻剂结肠组饲以含酚酞饲料。通过RT-qPCR及Western blot法对阿片受体在结肠中的表达进行相对定量分析。结果 3种阿片受体亚型在泻剂结肠组及对照组大鼠结肠内均有不同程度的表达。RT-qPCR检测显示泻剂结肠组MOR、DOR及KOR mRNA相对表达水平均明显高于对照组[（5.093±1.410）

（1.092±0.222），（2.612±0.612）

（1.032±0.082），（2.607±0.327）

（0.995±0.109），

0.01

]

。Western blot检测显示泻剂结肠组MOR、DOR及KOR的表达较对照组明显升高[（0.361±0.023）

(0.152±0.021)，(0.246±0.059)

(0.159±0.005)，(0.309±0.018)

( 0.201±0.015)，

0.01

]

。结论各阿片受体亚型在泻剂结肠大鼠结肠中表达均明显增强，提示其对泻剂结肠大鼠结肠运动及分泌功能可能存在明显的抑制作用。

Abstract

Objective To determine the expression of 3 subtypes of opioid receptors

μ (mu-opioid receptor

MOR)

δ (delta-opioid receptor

DOR)

and κ (kappa-opioid receptor

KOR)

in the colon of rats with cathartic colon. Methods Twenty Wistar rats of either sex at an age of 7 to 6 weeks

weighing 200±20 g were randomly divided into control group (

=10

fed with normal chow) and cathartic colon group [

=10

fed with 3 cycles of 200 mg/(kg·d) phenolphthalein first and then increased by this dose till 80% of rats having loose stool disappeared

]

. RT-qPCR and Western blotting were adopted to detect the expression of the 3 subtypes of opioid receptors. Results All 3 subtypes of opioid receptor were expressed distinctly in the colon between cathartic group and control group. RT-qPCR indicated that the expression of MOR

DOR and KOR at mRNA levels were significantly higher in cathartic colon group than in control group (5.093±1.410

1.092±0.222

2.612±0.612

1.032±0.082

2.607±0.327

0.995±0.109

0.01). While Western blot analysis showed their protein levels were in consistence with their mRNA levels between the 2 groups (0.361±0.023

s 0.152±0.021

0.246±0.059

0.159±0.005

0.309±0.018 vs 0.201±0.015

0.01)

showing that all 3 subtypes of opioid receptor expressed more intensively（

0.001）. Conclusion Three subtypes of opioid receptors are enhanced in the cathartic colon

suggesting that colonic motility and secretion are significantly suppressed in slow transit constipation.

关键词

阿片受体便秘泻剂结肠表达大鼠

Keywords

opioid receptorconstipationcatharticscolonexpressionrats

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