Inflammatory stress exacerbates cholesterol import in fatty liver of CCl<sub>4</sub>-induced mice <em>via</em> LDLr-SREBP-2-SCAP pathway

Tian Lin; Liu Hong; Xie Youhong

您当前的位置：

首页 >

文章列表页 >

Inflammatory stress exacerbates cholesterol import in fatty liver of CCl₄-induced mice via LDLr-SREBP-2-SCAP pathway

更新时间：2025-01-15

- Inflammatory stress exacerbates cholesterol import in fatty liver of CCl₄-induced mice via LDLr-SREBP-2-SCAP pathway
- Journal of Army Medical University Vol. 36, Issue 11, Pages: 1158-1162(2014)
- 作者机构：
  
  1. 重庆医科大学附属第一医院：消化内科，生殖健康与不孕专科
  2. 重庆医科大学附属大学城医院消化中心
- 作者简介：
- 基金信息：
- DOI：
  CLC： R363;R364.5;R575.5
- Published：2014，
  
  Published Online：15 June 2014，
- 稿件说明：
移动端阅览
TIAN LIN, LIU HONG, XIE YOUHONG. Inflammatory stress exacerbates cholesterol import in fatty liver of CCl₄-induced mice via LDLr-SREBP-2-SCAP pathway. [J]. Journal of army medical university, 2014, 36(11): 1158-1162.
DOI：

TIAN LIN, LIU HONG, XIE YOUHONG. Inflammatory stress exacerbates cholesterol import in fatty liver of CCl₄-induced mice via LDLr-SREBP-2-SCAP pathway. [J]. Journal of army medical university, 2014, 36(11): 1158-1162. DOI：

摘要

目的探讨炎症促进CCl

所致的单纯性脂肪变肝细胞进一步脂质集聚形成脂肪肝的分子机制。方法将12只6~8周龄的 C57BL/6J雄性小鼠采用随机数字表法分为对照组（

=6）和炎症组（

=6）。2组均预先皮下注射CCl

2周，再分别给予对照组和炎症组生理盐水和酪蛋白皮下注射16周，18周后留取小鼠血清及肝组织，检测血清炎症因子及脂质水平，HE及油红O染色观察小鼠脂肪肝病变程度，RT-PCR、免疫组化及Western blot分别检测小鼠肝组织的低密度脂蛋白受体（low-density lipoprotein receptor，LDLr）、固醇调节元件结合蛋白-2（sterol regulatory element binding protein 2

SREBP-2）和SREBP裂解激活蛋白（SREBP cleavage activating protein，SCAP）的基因和蛋白表达水平。结果炎症组的血清淀粉样蛋白（serum amyloid A

SAA）和TNF-α的表达均较对照组明显升高[分别为(236.09±38.45)ng/mL

(35.68±11.34)ng/mL

(82.59±11.48)pg/mL

(17.91±2.77)pg/mL

0.05

]

，炎症组的血清HDL-c、LDL-c、TC均较对照组明显降低[分别为(0.91±0.10)

(1.80±0.12)mmol/L

(2.65±0.44)

(5.76±0.33)mmol/L

(3.69±0.26)

(4.16±0.22)mmol/L

0.05

]

HE及油红O染色显示炎症组可见大量的脂质堆积。炎症组的LDLr、SREBP-2、SCAP的mRNA和蛋白表达比对照组明显增高(

0.05)。结论炎症可能通过上调肝细胞LDLr、SREBP-2、SCAP的表达，导致CCl

刺激下的脂肪变肝细胞摄入外源性胆固醇增加，加重肝脏脂质的异常集聚。

Abstract

Object

ive To investigate the molecular mechanism of inflammatory stress promoting the simple steatosis caused by carbon tetrachloride (CCl

) to further lipid accumulation. Methods A total of 12 male C57BL/6J mice at 6 to 8 weeks were randomly divided into control group (

=6) and inflammation group (

=6). In 2 weeks after subcutaneous treatment of CCl

the animals from control and inflammation groups were given normal saline and casein respectively by subcutaneous injection for 16 weeks. At the end of the treatment

the blood sample and liver tissue of the mice were collected. Serum levels of lipids were detected by enzymatic assay. Inflammatory cytokines

such as serum amyloid A (SAA) and tumor necrosis factor-α (TNF-α) were determined by ELISA. Lipid accumulation in the liver was evaluated by HE staining and oil red O staining. The expression of low-density lipoprotein receptor (LDLr)

sterol regulatory element binding protein-2 (SREBP-2) and SREBP cleavage activating protein (SCAP) at mRNA and protein levels were analyzed by real-time polymerase chain reaction (RT-PCR)

immunohistochemistry staining and Western blotting respectively. Results The serum levels of SAA and TNF-α were significantly higher in inflammation group than in the control group (236.09±38.45

35.68±11.34 ng/mL

82.59±11.48

17.91±2.77 pg/mL

both

0.05). Casein treatment resulted in significantly declines in the serum levels of HDL-c

LDL-c and TC when compared with the control group (0.91±0.10

1.80±0.12 mmol/L

2.65±0.44

5.76±0.33 mmol/L

3.69±0.26

4.16±0.22 mmol/L

all

0.05). HE staining and oil red O staining indicated that a lot of lipid accumulation were observed in the liver of mice in inflammation group. The mRNA and protein expression levels of LDLr

SREBP-2 and SCAP were significantly increased in inflammation group than in control mice (

0.05). Conclusion Inf

lammation may exacerbate lipid accumulation

via

up-regulating the expression of LDLr

SREBP-2 and SCAP in the fatty liver of C57BL/6J mice induced by CCl

关键词

炎症四氯化碳SREBP-2LDLrSCAP

Keywords

inflammationcarbon tetrachloridesterol regulatory element binding protein-2low-density lipoprotein receptorSREBP cleavage activating protein

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

CHEN Xiongying

LU Cheng

DONG Hui

XU Feng

CEN Ruiyan

LI Lingfei

DENG Yumeng

HE Yongqing

Related Institution

Pediatric Intensive Care Unit, National Clinical Research Center for Child Health and Disorders, Key Laboratory of Child Development and Disorders of Ministry of Education, Chongqing Key Laboratory of Pediatrics, Children’s Hospital of Chongqing Medical University

Department of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University)

Department of Dermatology, Army Medical Center of PLA

Department of Anesthesiology, First Affiliated Hospital, Army Medical University(Third Military Medical University)

Faculty of Traditional Chinese Medicine, 2Faculty of Pharmacy, Dazhou Vocational College of Traditional Chinese Medicine

⁰

Inflammatory stress exacerbates cholesterol import in fatty liver of CCl4-induced mice via LDLr-SREBP-2-SCAP pathway

DOI：

摘要

Abstract

关键词

Keywords

references

Inflammatory stress exacerbates cholesterol import in fatty liver of CCl₄-induced mice via LDLr-SREBP-2-SCAP pathway