Objective　To explore the method and effect of applying just-in-time-teaching (JiTT) mode in the reform of the teaching method of biochemical comprehensive experiment (Isolation

Purification and Identification of γ-Globulin From serum). Methods　A total of 100 undergraduate students in our 2016 clinical medicine were divided evenly into two groups: the control group and the experimental group. The control group used traditional teaching

and the experimental group used JiTT-based teaching. In the experimental group

an online environment for pre-classroom discussion and feedback was constructed by uploading learning resources such as short video and tutorials to the online teaching platform and the online learning exchange group 3-5 days before the class; the class session itself consisted of personal tests

face-to-face teaching by teachers

group discussion

finishing experimental report and answering questions etc.

and was intended to evaluate the students' self-study. SPSS 22.0 was used to collect exam scores and questionnaire results

and the data between groups were compared using t test. Results　The scores of individual test scores in the experimental group (9.44±0.59) were higher than those in the control group (8.77±0.41) (P=0.00)

and the difference was statistically significant. In all the six questionnaire items pertaining to teaching effect

JiTT teaching method received higher scores than the traditional teaching method (P<0.05)

and the difference was statistically significant. Conclusion　JiTT can improve the quality of biochemistry experiment teaching. It is worthwhile to try it in the teaching of other basic specialized courses.