Objective　To evaluate the role of evidence-based nursing in clinical ophthalmology teaching. Methods　Fifty nursing interns in department of ophthalmology in Southwest Hospital were randomized into control group and observation group in accordance with the principle of equal number of junior college students and undergraduates. Traditional teaching was applied in control group while clinical ophthalmology teaching combined with traditional teaching was used in observation group. Teaching effect was evaluated at the end of internship by questionnaire and examination. Data were analyzed by SPSS 13.0 using χ2　test and independent sample t test（taking students with score ≥60 as effective indicator）. P < 0.05 indicated significant difference. Results　Scores of questionnaire and examination were higher in observation group than in control group， with statistically significant differ-ence（ χ2=6.818， P=0.018）. Theoretical exam score was 67.12±11.53 in control group and 84.96±9.27 in observation group； practical score was 65.68±8.0 in control group and 80.28±10.65 in observation group with significant differences between two groups（P=0.000）. Conclusions　Adoption of evidence-based nursing intervention in ophthalmic clinical teaching help students learn to think，consult literature and use critical thinking to raise questions during the internship. It also makes stu-dents regard the results of scientific research and personal experience as the basis for mastering pro-fessional and scientific expertise， thereby enhancing the ability of nursing practice.