Objective　To study the status of professional commitment and its effect on self-esteem of non-medical undergraduates in medical colleges. Methods　Totally 410 non-medical under-graduates and 403 medical undergraduate were investigated by professional commitment of undergrad-uate scales and self-esteem questionnaire of undergraduates. T test， chi-square test， ANOVA， corre-lation analysis and multiple regression analysis were employed to do statical analysis， with P＜0.05 be-ing statistical differences. Results　Non-medical undergraduates’ scores of professional commitment （85.11±11.60） and self-esteem （2.57±0.37） were lower than those （93.82±11.43） and （2.62±0.35） of medical undergraduates. There were statistical differences in non-medical undergraduates’ professional commitment concerning the variables of professional consistency （t=5.657， P=0.000） and grades （F=8.528， P=0.000）. Professional commitment of non-medical undergraduates was positively correlated with self-esteem. There was linear regression relationship between the variables of emotion-al commitment （P=0.005）， normative commitment （P=0.006） and self-esteem. Conclusions　Non-medical undergraduates' professional commitment and self-esteem levels are lower than those of medical undergraduates. The professional commitment could produce obvious effects on self-esteem.