Objective　To investigate the application efficacy objective structured clinical exam-ination（OSCE） in assessment of interns of traditional Chinese medicine in anesthesiology department. Methods　Totally 62 interns were examed by OSCE formulated by anesthesiology department before de-partmental rotation. Contents of the exam included preoperative evaluation， endotracheal intubation tech-nique， deep venipuncture， lumbar puncture， case analysis， medical documents writing， etc. Assessment was made by exam results and questionnaire survey. Quantitative data were analyzed by ANOVA and results of questionnaire were analyzed by calculating percentage of each option and describing directly. Results　Test results showed that the score of medical document writing was the highest（18.25±1.53）， while that of case analysis was the lowest（13.49±1.46） and there was no significant difference in scores of three clinical skills. Questionnaire survey showed the OSCE method is superior to traditional method since it is more scientific， objective and fair. Conclusions　OSCE method can scientifically as-sess the clinical practice effect of interns of traditional Chinese medicine in anesthesiology department. Meanwhile， it can find the shortcomings in the teaching， which is helpful in promoting the assessment and improvement of clinical teaching.