Objective　To investigate the effect of PBL combined with EBM applied in the teaching of fever of unknown origin. Methods　PBL combined with EBM teaching was applied in fever of unknown origin course for 30 clinical medicine specialty（eight years） students of Tongji class of grade 2009（experiment group）， while PBL teaching was applied in fever of unknown origin course for 30 clinical medicine specialty（eight years） students of Tongji class of grade 2008（control group）. After teaching， the theory examination for both basic knowledge and case analysis was organized for all students of both groups. At the same time the questionnaire survey was conducted to 30 students of grade 2009 to evaluate the teaching effect. The results were assessed by using SPSS 18.0 statistical software for the T-test of the experimental group and the control group.Inspection level was ?琢=0.05. Results　The theory test score of students in the experimental group was （93.5±3.2） point， signifi-cantly higher than that of the students in the control group（84.7±2.8）. There was statistically signifi-cant difference between the scores of the two groups of students（P=0.00）. Survey results showed 19 students（63.33%） thought that the development of PBL teaching combined with evidence-based medicine teaching had its necessity， and 16 students（53.33% ） thought that the teaching method im-proved their clinical thinking ability of logical reasoning. Conclusion　The concept of PBL combined with EBM has achieved significant resultsinthe teaching offever of unknown origin， and it is necessary to carry out this teaching mode in medical colleges with certain teaching strength.