Objective　To explore the difference of hand hygiene requirement between guide-line of skill practice for national examinations of doctors' qualification（clinical practitioners） and stan-dard for hand hygiene for healthcare workers in health care settings for some sterile techniques. Methods　Eight sterile techniques in guideline of skill practice for national examinations of doctors' qualification（clinical practitioners） such as changing a dress and taking out stitches， aspiration， ure-thral catheterization， arteriovenous puncture， thoracentesis， abdominal puncture， lumbar puncture and bone marrow puncture were chosen and the results were compared with standard for hand hygiene for healthcare workers in health care settings. Results　Three of eight sterile technics mentioned hand-washing， six of eight mentioned wearing sterile gloves， and zero of eight mentioned antiseptic han-drubbing； however， only one of six（16.67%） before wearing sterile gloves mentioned handwashing. Conclusion　The requests of hand hygiene between guideline of skill practice for national examina-tions of doctors' qualification（clinical practitioners） and standard for hand hygiene for healthcare workers in healthcare settings are not concordant， which from the root leads to the inconsistence of doctors' learning at different stages of education and training. It is suggested that the examination organization departments should refer to the relevant provisions of the state in the preparation of guidelines， and regulate medical practice behavior with consistent standards.