【Abstract】 Endodontic treatment with the use of dental operating microscope is a difficult part in teaching. We applied cone beam computed tomography (CBCT) guided technology for microendodontic training of dental students who are in their 5th year of the 7 year course to pursue their master’s degree. The process of teaching is constituted of preoperative analysis，operation guided by CBCT ，postoperative therapeutic evaluation. And the result of teaching quality is acquired by questionnaire. This method improved student’s capacities of analysis and solution in intractable cases and greatly motivated students’ participation，as well as promoting their learning efficiency. The application of this technique in teaching process compensates the deficiency of traditional teaching method by shaking off the fetters of experience-dependent pattern in the endodontic microscope teaching ，and is worth to be popularized in endodontic education .