IMMUNODIAGNOSIS OF AMOEBIASIS BY INDIRECT IMMUNOPEROXIDASE ASSAY

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IMMUNODIAGNOSIS OF AMOEBIASIS BY INDIRECT IMMUNOPEROXIDASE ASSAY

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- IMMUNODIAGNOSIS OF AMOEBIASIS BY INDIRECT IMMUNOPEROXIDASE ASSAY
- Journal of Chongqing Medical University Issue 2, Pages: 103-107(1985)
- 作者机构：
  
  1. 重庆医学院第一医院传染病学教研室
  2. 北京热带医学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：1985，
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[1]陈雅棠,刘约翰,王其南,马映雪,余登高,郭增柱,王正仪,桂淑环.间接免疫过氧化物酶试验诊断阿米巴病[J].重庆医学院学报,1985(02):103-107.

IMMUNODIAGNOSIS OF AMOEBIASIS BY INDIRECT IMMUNOPEROXIDASE ASSAY. [J]. Journal of Chongqing Medical University (2):103-107(1985)
[1]陈雅棠,刘约翰,王其南,马映雪,余登高,郭增柱,王正仪,桂淑环.间接免疫过氧化物酶试验诊断阿米巴病[J].重庆医学院学报,1985(02):103-107. DOI：

IMMUNODIAGNOSIS OF AMOEBIASIS BY INDIRECT IMMUNOPEROXIDASE ASSAY. [J]. Journal of Chongqing Medical University (2):103-107(1985) DOI：

摘要

用间接免疫过氧化物酶试验（ⅡP）对15例急性阿米巴病人血清特异性抗体进行了检测

12例阿米巴肝脓肿病人全部阳性

滴度分布为1:40～1:640;3例急性阿米巴痢疾病人亦全部阳性

但滴度较低

为1:40～1:160。本法与间接荧光抗体试验和酶联免疫吸附试验相比

阳性率无显著差异。ⅡP操作简单

不须荧光显微镜及阿米巴纯培养

有较好的特异性

故适于普通实验室用于阿米巴病的诊断。

Abstract

The indirect immunoperoxidase assay (IIP) was evaluated for the detection of specific antibodies in sera from 15 cases of acute atriebiasis. 12 cases of amebic liver abscess were all positive for IIP assay

the titres ranged from 1:40 to 1 = 640; 3 cases of acute amebic dysentery were also positive for IIP assay

but their titres were comparatively lower and ranged from 1:40 to 1:160. Compared with the results of IFA and ELISA

no significant difference was found. IIP assay is easy to perform

does not require fluorescent microscope and amebic axenic culture.and has good specifity. Therefore

this method can be used for the diagnosis of acute amebiasis in a general laboratory.

关键词

阿米巴间接免疫过氧化物酶试验

Keywords

AmoebiasisIndirect immunoperoxidase assay

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