It is quite difficult to culture leukemic bone marrow cells for sister chromatid exchange (SCE) detection. As yet

there were a few about it in the literature. We reported a simple

quick method of SCE detection in leukemic bone marrow cells under gerneral laboratory conditions. It needed only 52 hours for culture. Giemss stain was used in differential staining without fluorescent dys.We observed the SCE incidence

growth kinetic and Ph’ positive rates in 10 normal persons and 7 patients with untreated CML with this method. The results were similar with other reports.It was the two succesfull factors of this mehtod that the culture cells concertration were severely controled and the reparation of culture medium was modified