STUDIES ON EARLY DIAGNOSIS OF MDS BY CLONAL ANALYSIS  USING PCR TECHNIQUE AND X-LINKED RFLP-METHYLATLON ANALYSIS

Zhang ling

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STUDIES ON EARLY DIAGNOSIS OF MDS BY CLONAL ANALYSIS USING PCR TECHNIQUE AND X-LINKED RFLP-METHYLATLON ANALYSIS

更新时间：2024-01-17

- STUDIES ON EARLY DIAGNOSIS OF MDS BY CLONAL ANALYSIS USING PCR TECHNIQUE AND X-LINKED RFLP-METHYLATLON ANALYSIS
- Journal of Chongqing Medical University Issue 4, (1994)
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  CLC： R394.8
- Published：1994，
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[1]张玲.克隆分析用于MDS早期诊断的初步研究──用PCR技术和x染色体联锁的PGK基因RFLP及甲基化的分析法[J].重庆医科大学学报,1994(04):270-273.

Zhang ling. STUDIES ON EARLY DIAGNOSIS OF MDS BY CLONAL ANALYSIS USING PCR TECHNIQUE AND X-LINKED RFLP-METHYLATLON ANALYSIS. [J]. Journal of Chongqing Medical University (4).(1994)
[1]张玲.克隆分析用于MDS早期诊断的初步研究──用PCR技术和x染色体联锁的PGK基因RFLP及甲基化的分析法[J].重庆医科大学学报,1994(04):270-273. DOI：

Zhang ling. STUDIES ON EARLY DIAGNOSIS OF MDS BY CLONAL ANALYSIS USING PCR TECHNIQUE AND X-LINKED RFLP-METHYLATLON ANALYSIS. [J]. Journal of Chongqing Medical University (4).(1994) DOI：

摘要

用ＰＣＲ技术和ｘ染色体联锁的ＰＧＫ基因的ＲＦＬＰ及甲基化分析方法，分别对３００例老年、２００例中青年、无血液系统疾病的女性进行了外周血细胞的克隆研究。ＰＧＫ基因的ＲＦＬＰ检出率老年组为３２．７％，中青年组为３２．５％。克隆分析：中青年组均为多克隆，老年组发现５例有克隆变异，其中一例随访发现有外周血细胞异常，疑为ＭＤＳ。此方法可能对ＭＤＳ的早期诊断有一定价值。

Abstract

lonal study of peripheral blood cells in 300 elderly and 200 middle-aged females without haematologicaldisorders was performed by PcR technique and X-linked RFLP of PGK gene-methylation analysis. The fre-quency of the PGK of polymorphism was 32.7%and 32.5%in elderly and middle-aged groups respectively

Clonal hacmopoiesis was not fourd in middel-aged group

but could be seen in 5 individuals of eldefly groupand one of them has show the abnormakuty of peripheral blood cells as MDS during following up.The resultsuggests this study may provide early information on sub-clinical malignant changes.

关键词

骨髓增生异常综合征PCR磷酸甘油酸激酶限制性片段长度多态性

Keywords

Myelodysplastic syndromePolymerase chain reactionPhsophoglycerate kinase geneRe-striction fragment length polymorphism

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Department of Hematology,the Second Affiliated Hospital,Chongqing Medical University

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